Increased CD123 + HLA-DR- Granulocytes in Allergic Rhinitis and Influence of Allergens on Expression of Cell Membrane Markers.

IF 2.5 3区 医学 Q1 OTORHINOLARYNGOLOGY American Journal of Rhinology & Allergy Pub Date : 2024-09-01 Epub Date: 2024-05-07 DOI:10.1177/19458924241252456
Hua Xie, Huiyun Zhang, Dong Chen, Lei Cheng, Fangqiu Gu, Shunlan Wang, Meicen Liu, Li Li, Qingwei Zeng, Shaoheng He
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Abstract

Background: It is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes.

Objective: To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.

Methods: Flow cytometry was used to detect the expression of the membrane molecules.

Results: While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.

Conclusions: The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.

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过敏性鼻炎中 CD123 + HLA-DR- 粒细胞的增加及过敏原对细胞膜标记表达的影响
背景:据报道,PBMC 中的 CD123 + HLA-DR- 细胞是嗜碱性粒细胞,CD203c、CD63 和 FcεRI 分子是嗜碱性粒细胞的活化标记。然而,人们对血液粒细胞中的 CD123 + HLA-DR 细胞知之甚少:目的:研究过敏性鼻炎(AR)患者血粒细胞和外周 PBMC 中 CD123 + HLA-DR- 细胞的存在,以及过敏原对嗜碱性粒细胞细胞膜标记的影响:方法:采用流式细胞术检测膜分子的表达:结果:虽然 CD123 + HLA-DR- PBMCs 是嗜碱性粒细胞的代表,但它们的存在在 AR 患者中没有明显变化。与此相反,CD123 + HLA-DR- 粒细胞(仅占嗜碱性粒细胞的 50%)在季节性(sAR)和常年性(pAR)AR 患者中的比例和数量均显著增加。CD123+HLA-DR-粒细胞中的CD63+、CD203c+和FcεRIα+细胞在AR患者中也显示出更强的活性。从屋尘螨过敏原提取物(HDME)和野蒿提取物中提取的过敏原进一步增加了sAR和pAR患者粒细胞中CD123 + HLA-DR-细胞的数量,也增加了pAR患者PBMCs中CD123 + HLA-DR-细胞的数量:结论:使用 CD123 + HLA-DR- 粒细胞和 PBMC 可能不足以诊断 AR。过敏原可能会影响 CD123 + HLA-DR- 细胞的数量以及这些细胞中 CD63、CD203c 和 FcεRIα 的表达,从而导致 AR 的发生。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
82
审稿时长
4-8 weeks
期刊介绍: The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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