Effect of bile reflux on gastric juice microbiota in patients with different histology phenotypes.

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Gut Pathogens Pub Date : 2024-05-07 DOI:10.1186/s13099-024-00619-7

Yong Sung Kim, Tatsuya Unno, Seon-Young Park, Jin Ook Chung, Yoo-Duk Choi, Su-Mi Lee, Seong Hyun Cho, Dong Hyun Kim, Hyun-Soo Kim, Young Do Jung

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Abstract

Background/aims: Bile reflux (BR) can influence the gastric environment by altering gastric acidity and possibly the gastric microbiota composition. This study investigated the correlation between bile acids and microbial compositions in the gastric juice of 50 subjects with differing gastric pathologies.

Methods: This study included 50 subjects, which were categorized into three groups based on the endoscopic BR grading system. The primary and secondary bile acid concentrations in gastric juice samples were measured, and microbiota profiling was conducted using 16 S rRNA gene sequencing.

Results: Significant differences were observed in each bile acid level in the three endoscopic BR groups (P < 0.05). The Shannon index demonstrated a significant decrease in the higher BR groups (P < 0.05). Analysis of the β-diversity revealed that BR significantly altered the gastric microbiota composition. The presence of neoplastic lesions and the presence of H. pylori infection impacted the β-diversity of the gastric juice microbiota. The abundance of the Streptococcus and Lancefielfdella genera exhibited positive correlations for almost all bile acid components(P < 0.05). In addition, the abundance of Slobacterium, Veillonella, and Schaalia showed positive correlations with primary unconjugated bile acids (P < 0.05).

Conclusion: Changes in microbial diversity in the gastric juice were associated with BR presence in the stomach. This result suggests that the degree of BR should be considered when studying the gastric juice microbiome.

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胆汁反流对不同组织学表型患者胃液微生物群的影响。

背景/目的：胆汁反流（BR）可通过改变胃酸度来影响胃环境，还可能改变胃微生物群的组成。本研究调查了 50 名患有不同胃病的受试者胃液中胆汁酸和微生物组成之间的相关性：本研究纳入了 50 名受试者，根据内镜 BR 分级系统将其分为三组。测量了胃液样本中的一级和二级胆汁酸浓度，并使用 16 S rRNA 基因测序法进行了微生物群谱分析：结果：在三个内镜下 BR 组中，各胆汁酸水平均存在显著差异（P 结论：内镜下 BR 组的胆汁酸水平较低，而内镜下 BR 组的胆汁酸水平较高：胃液中微生物多样性的变化与胃中是否存在胆汁酸有关。这一结果表明，在研究胃液微生物组时应考虑胆汁酸的程度。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).