Hemolymph metabolism of black soldier fly (Diptera: Stratiomyidae), response to different supplemental fungi.

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY Journal of Insect Science Pub Date : 2024-05-01 DOI:10.1093/jisesa/ieae050
Mani Kannan, Tzach Vitenberg, Ron Schweitzer, Itai Opatovsky
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Abstract

The black soldier fly, Hermetia illucens L. (Diptera: Stratiomyidae), is commonly used for organic waste recycling and animal feed production. However, the often inadequate nutrients in organic waste necessitate nutritional enhancement of black soldier fly larvae, e.g., by fungal supplementation of its diet. We investigated the amino acid composition of two fungi, Candida tropicalis (Castell.) Berkhout (Saccharomycetales: Saccharomycetaceae) and Pichia kudriavzevii Boidin, Pignal & Besson (Saccharomycetales: Pichiaceae), from the black soldier fly gut, and commercial baker's yeast, Saccharomyces cerevisiae Meyen ex E.C. Hansen (Saccharomycetales: Saccharomycetaceae), and their effects on larval growth and hemolymph metabolites in fifth-instar black soldier fly larvae. Liquid chromatography-mass spectrometry was used to study the effect of fungal metabolites on black soldier fly larval metabolism. Amino acid analysis revealed significant variation among the fungi. Fungal supplementation led to increased larval body mass and differential metabolite accumulation. The three fungal species caused distinct metabolic changes, with each over-accumulating and down-accumulating various metabolites. We identified significant alteration of histidine metabolism, aminoacyl-tRNA biosynthesis, and glycerophospholipid metabolism in BSF larvae treated with C. tropicalis. Treatment with P. kudriavzevii affected histidine metabolism and citrate cycle metabolites, while both P. kudriavzevii and S. cerevisiae treatments impacted tyrosine metabolism. Treatment with S. cerevisiae resulted in down-accumulation of metabolites related to glycine, serine, and threonine metabolism. This study suggests that adding fungi to the larval diet significantly affects black soldier fly larval metabolomics. Further research is needed to understand how individual amino acids and their metabolites contributed by fungi affect black soldier fly larval physiology, growth, and development, to elucidate the interaction between fungal nutrients and black soldier fly physiology.

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黑兵蝇(双翅目:Stratiomyidae)的血淋巴代谢,对不同补充真菌的反应。
黑实蝇(Hermetia illucens L.)(双翅目:实蝇科)通常用于有机废物回收和动物饲料生产。然而,有机废物中的营养成分往往不足,因此有必要通过在食物中添加真菌等方法来增强黑翅大实蝇幼虫的营养。我们研究了黑实蝇肠道中的两种真菌--热带念珠菌(Castell. Berkhout)(酵母菌纲:Saccharomycetales: Saccharomycetaceae)和毕赤霉(Pichia kudriavzevii Boidin, Pignal & Besson)(酵母菌纲:Pichiaceae),以及商业面包酵母--酿酒酵母(Saccharomyces cerevisiae Meyen ex E. C. Hansen)(酵母菌纲:Saccharomycetales: Pichiaceae)的氨基酸组成。C. Hansen(Saccharomycetales: Saccharomycetaceae),以及它们对五龄黑刺蝇幼虫生长和血淋巴代谢物的影响。利用液相色谱-质谱法研究了真菌代谢物对黑实蝇幼虫新陈代谢的影响。氨基酸分析表明,不同真菌之间存在显著差异。补充真菌会导致幼虫体重增加和不同代谢物的积累。三种真菌引起了不同的代谢变化,每种真菌都过度积累和减少积累各种代谢物。我们发现,在用热带真菌处理的 BSF 幼虫体内,组氨酸代谢、氨基酰-tRNA 生物合成和甘油磷脂代谢发生了明显变化。用 P. kudriavzevii 处理会影响组氨酸代谢和柠檬酸循环代谢产物,而 P. kudriavzevii 和 S. cerevisiae 处理则会影响酪氨酸代谢。用 S. cerevisiae 处理会导致与甘氨酸、丝氨酸和苏氨酸代谢有关的代谢物积累减少。这项研究表明,在幼虫食物中添加真菌会显著影响黑翅大实蝇幼虫的代谢组学。需要进一步研究真菌提供的单个氨基酸及其代谢产物如何影响黑翅大实蝇幼虫的生理、生长和发育,以阐明真菌营养物质与黑翅大实蝇生理之间的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Insect Science
Journal of Insect Science 生物-昆虫学
CiteScore
3.70
自引率
0.00%
发文量
80
审稿时长
7.5 months
期刊介绍: The Journal of Insect Science was founded with support from the University of Arizona library in 2001 by Dr. Henry Hagedorn, who served as editor-in-chief until his death in January 2014. The Entomological Society of America was very pleased to add the Journal of Insect Science to its publishing portfolio in 2014. The fully open access journal publishes papers in all aspects of the biology of insects and other arthropods from the molecular to the ecological, and their agricultural and medical impact.
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