Evaluation of the Influence of Chromium and Zinc Ions on the Collagenolysis Process in Solutions by the Method of Dynamic Light Scattering

IF 0.8 4区 物理与天体物理 Q4 OPTICS Optics and Spectroscopy Pub Date : 2024-04-01 DOI:10.1134/S0030400X23100181
A. V. Petrova, A. V. Sidorova, I. A. Sergeeva, G. P. Petrova
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Abstract

Using the method of dynamic light scattering, it was found that the rate of collagen cleavage under the influence of bacterial collagenase decreases by almost 4 times when protein molecules are doped with Cr3+ ions, and when the enzyme activator of Zn2+ ions is added, it increases by 1.3 times. Based on the experimental data, the reaction rate constants k1 were calculated. It was found that when collagen molecules are tanned with a chromium salt, it becomes more resistant to degradation in solutions containing zinc ions than in solutions with the addition of calcium ions, as we described in earlier works.

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用动态光散射法评估铬和锌离子对溶液中胶原蛋白溶解过程的影响
摘要 利用动态光散射的方法发现,当蛋白质分子中掺入 Cr3+ 离子时,在细菌胶原酶作用下胶原蛋白的裂解速率降低了近 4 倍,而当加入 Zn2+ 离子的酶活化剂时,则增加了 1.3 倍。根据实验数据,计算了反应速率常数 k1。研究发现,当胶原蛋白分子被铬盐鞣制后,在含有锌离子的溶液中比在添加了钙离子的溶液中更耐降解,这一点我们在之前的工作中已有描述。
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来源期刊
Optics and Spectroscopy
Optics and Spectroscopy 物理-光谱学
CiteScore
1.60
自引率
0.00%
发文量
55
审稿时长
4.5 months
期刊介绍: Optics and Spectroscopy (Optika i spektroskopiya), founded in 1956, presents original and review papers in various fields of modern optics and spectroscopy in the entire wavelength range from radio waves to X-rays. Topics covered include problems of theoretical and experimental spectroscopy of atoms, molecules, and condensed state, lasers and the interaction of laser radiation with matter, physical and geometrical optics, holography, and physical principles of optical instrument making.
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