Cell Cycle Parameters and Ornithine Decarboxylase Activity in the Red Bone Marrow of Hibernating Ground Squirrels Urocitellus undulatus

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/s0006350923050032

G. E. Aksyonova, O. S. Logvinovich, V. N. Afanasyev, K. I. Lizorkina

{"title":"Cell Cycle Parameters and Ornithine Decarboxylase Activity in the Red Bone Marrow of Hibernating Ground Squirrels Urocitellus undulatus","authors":"G. E. Aksyonova, O. S. Logvinovich, V. N. Afanasyev, K. I. Lizorkina","doi":"10.1134/s0006350923050032","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>During the hibernation season, the values for the parameters of the cell cycle of red bone marrow cells in the hibernating ground squirrels Urocitellus undulatus, when they return to an active-like state between periods of torpor and interbout arousal, do not differ from those observed in summer-active animals. In animals that enter a state of torpor, the cumulative percentage of cells in the resting phase (G0 phase) and pre-synthesis phase (G1 phase) increased from 71.8 to 76.0%, the percentage of cells in the synthesis phase (S phase) decreased from 19.3 to 15.3% compared to those animals that return to an active-like state between periods of torpor and interbout arousal. The cumulative percentage of cells in the post DNA synthesis phase (G2 phase) and mitosis (M) does not change, but (G2 + M)/S ratio increases. When animals enter a state of torpor, changes in parameter values are observed when the animal’s body temperature drops below 25°C; this effect refers to a system whose thermal relaxation time is a nonmonotonic function of the initial temperature. The activity of the key enzyme of polyamine synthesis ornithine decarboxylase, a marker of cell activation and proliferation during interbout arousal does not significantly differ from that observed in summer-active animals; the enzymatic activity decreases sharply, when animals decrease their body temperature below 25°C and enter the state of torpor, and this activity remains at a low level during hibernation and arousal until body temperature reaches 30°C. The role of changes in the parameter values associated with proliferative activity in adaptation of hematopoietic tissue during hibernation of the Yakutian ground squirrel is discussed.","PeriodicalId":493,"journal":{"name":"Biophysics","volume":null,"pages":null},"PeriodicalIF":4.0330,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://doi.org/10.1134/s0006350923050032","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

During the hibernation season, the values for the parameters of the cell cycle of red bone marrow cells in the hibernating ground squirrels Urocitellus undulatus, when they return to an active-like state between periods of torpor and interbout arousal, do not differ from those observed in summer-active animals. In animals that enter a state of torpor, the cumulative percentage of cells in the resting phase (G₀ phase) and pre-synthesis phase (G₁ phase) increased from 71.8 to 76.0%, the percentage of cells in the synthesis phase (S phase) decreased from 19.3 to 15.3% compared to those animals that return to an active-like state between periods of torpor and interbout arousal. The cumulative percentage of cells in the post DNA synthesis phase (G₂ phase) and mitosis (M) does not change, but (G₂ + M)/S ratio increases. When animals enter a state of torpor, changes in parameter values are observed when the animal’s body temperature drops below 25°C; this effect refers to a system whose thermal relaxation time is a nonmonotonic function of the initial temperature. The activity of the key enzyme of polyamine synthesis ornithine decarboxylase, a marker of cell activation and proliferation during interbout arousal does not significantly differ from that observed in summer-active animals; the enzymatic activity decreases sharply, when animals decrease their body temperature below 25°C and enter the state of torpor, and this activity remains at a low level during hibernation and arousal until body temperature reaches 30°C. The role of changes in the parameter values associated with proliferative activity in adaptation of hematopoietic tissue during hibernation of the Yakutian ground squirrel is discussed.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

冬眠地松鼠红骨髓中的细胞周期参数和鸟氨酸脱羧酶活性

摘要在冬眠季节，冬眠地鼠（Urocitellus undulatus）的红骨髓细胞周期参数值在冬眠期和间歇唤醒期之间恢复到类似活动状态时，与在夏季活动的动物中观察到的参数值没有差异。与在休眠期和趾间唤醒期之间恢复活动状态的动物相比，进入休眠状态的动物处于静止期（G0 期）和合成前期（G1 期）的细胞累积百分比从 71.8% 增加到 76.0%，处于合成期（S 期）的细胞百分比从 19.3% 下降到 15.3%。处于 DNA 合成后阶段（G2 阶段）和有丝分裂阶段（M 阶段）的细胞累积百分比没有变化，但（G2 + M）/S 比率增加了。当动物进入冬眠状态时，当动物体温降至 25°C 以下时，会观察到参数值的变化；这种效应是指系统的热弛豫时间是初始温度的非单调函数。多胺合成的关键酶鸟氨酸脱羧酶是间歇性唤醒期间细胞活化和增殖的标志，其活性与夏季活动的动物没有明显差异；当动物体温降至 25°C 以下并进入冬眠状态时，酶活性急剧下降，在冬眠和唤醒期间，酶活性保持在较低水平，直到体温达到 30°C。本文讨论了雅库特地松鼠冬眠期间与增殖活动相关的参数值变化在造血组织适应过程中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.