Microtubule-Associated Protein 9 Is a Potential Tumor Suppressor That Is Inactivated by Methylation in Cervical Cancer

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2024-05-02 DOI:10.1134/s0026893324700304
P. Li, F. Wang, Y. Y. Gao, W. Zhang, Y. Zhang
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Abstract

Cervical cancer is a malignant disease that seriously affects women’s health, and the cause of cervical cancer is complex. Microtubule-associated protein 9 (MAP9) localizes to the mitotic spindle and it plays an important role in spindle assembly and centrosome integrity maintenance. The expression and function of MAP9 is unclear in cervical cancer. This study aims to explore the relationship between DNA methylation of MAP9 and cervical cancer, and the function of MAP9 in cervical cancer. qRT-PCR was used to detect the transcriptional expression of MAP9 in cervical cancer tissues and cell lines. The methylation status of MAP9 promoter was determined by bisulfite sequencing PCR (BSP). The DNA methyltransferase inhibitor, 5-Aza-CdR, was used to inhibit the activity of methyltransferase. Then, the cell proliferation was performed by CCK-8 assay after the transfection of MAP9 plasmids. Furthermore, the cell cycle and apoptosis were performed by flow cytometry. It was found that MAP9 was down-regulated both in cervical cancer tissues and cell lines. The promoter and the first exon region of MAP9 was hypermethylated and its mRNA expression could be restored after the treatment of 5-Aza-CdR. Ectopic expression of MAP9 could inhibit cell proliferation and cell cycle, without effecting the cell apoptosis. The low expression of MAP9 in cervical cancer dues to its hypermethylation and acts a potential tumor suppressor gene. MAP9 might be a novel biomarker in cervical cancer.

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微管相关蛋白 9 是一种潜在的肿瘤抑制因子,在宫颈癌中因甲基化而失活
摘要 宫颈癌是严重影响妇女健康的恶性疾病,其病因复杂。微管相关蛋白9(MAP9)定位于有丝分裂纺锤体,在纺锤体组装和中心体完整性维护中发挥着重要作用。MAP9在宫颈癌中的表达和功能尚不清楚。本研究旨在探讨MAP9的DNA甲基化与宫颈癌的关系,以及MAP9在宫颈癌中的功能。通过亚硫酸氢盐测序 PCR(BSP)测定 MAP9 启动子的甲基化状态。DNA 甲基转移酶抑制剂 5-Aza-CdR 可抑制甲基转移酶的活性。转染 MAP9 质粒后,用 CCK-8 检测细胞增殖。此外,还用流式细胞术检测了细胞周期和细胞凋亡。结果发现,宫颈癌组织和细胞系中的 MAP9 均出现下调。经 5-Aza-CdR 处理后,MAP9 的启动子和第一外显子区发生了高甲基化,其 mRNA 表达得以恢复。异位表达的MAP9可抑制细胞增殖和细胞周期,但不影响细胞凋亡。MAP9在宫颈癌中的低表达是其高甲基化的结果,它是一种潜在的抑癌基因。MAP9可能是宫颈癌的一种新型生物标志物。
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来源期刊
Molecular Biology
Molecular Biology 生物-生化与分子生物学
CiteScore
1.30
自引率
8.30%
发文量
78
审稿时长
3 months
期刊介绍: Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.
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