An effective and comprehensive optimization strategy for preparing Ginkgo biloba leaf extract enriched in shikimic acid by macroporous resin column chromatography.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS Phytochemical Analysis Pub Date : 2024-08-01 Epub Date: 2024-05-09 DOI:10.1002/pca.3375
Sijie Zhang, Xingchu Gong, Haibin Qu
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Abstract

Introduction: Previously reported preparation methods of Ginkgo biloba leaf extract (EGBL) have mainly focused on the enrichment of flavonoid glycosides (FG) and terpene trilactones (TT), which led to the underutilization of G. biloba leaves (GBL).

Objectives: To make full use of GBL, in this study, a comprehensive optimization strategy for preparing EGBL by macroporous resin column chromatography was proposed and applied to enrich FG, TT, and shikimic acid (SA) from GBL.

Methodology: Initially, the static adsorption and desorption were executed to select suitable resin. Then, the influences of solution pH were investigated by the static and dynamic adsorption. Subsequently, eight process parameters were systematically investigated via a definitive screening design (DSD). After verification experiments, scale-up enrichment was carried out, investigating the feasibility of the developed strategy for application on an industrial scale.

Results: It was found that XDA1 was the most appropriate adsorbent for the preparation of EGBL at solution pH 2.0. Furthermore, based on the constraints of the desired quality attributes, the optimized ranges of operating parameters were successfully acquired, and the verification experiments demonstrated the accuracy and reliability of using DSD to investigate the chromatography process for the preparation of EGBL. Finally, magnified experiments were successfully performed, obtaining the EGBL containing 26.54% FG, 8.96% TT, and 10.70% SA, which reached the SA level of EGB761, an international standard EGBL.

Conclusion: The present study not only provided an efficient and convenient approach for the preparation of EGBL enriched in SA but also accelerated efforts to high-value utilization of GBL.

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用大孔树脂柱层析法制备富含莽草酸的银杏叶提取物的有效综合优化策略
简介:以往报道的银杏叶提取物(EGBL)的制备方法主要集中在黄酮苷(FG)和萜烯三内酯(TT)的富集上,导致银杏叶(GBL)的利用率不足:为了充分利用 GBL,本研究提出了大孔树脂柱层析制备 EGBL 的综合优化策略,并将其应用于从 GBL 中富集 FG、TT 和莽草酸(SA):首先,通过静态吸附和解吸选择合适的树脂。然后,通过静态和动态吸附研究了溶液 pH 值的影响。随后,通过确定性筛选设计(DSD)对八个工艺参数进行了系统研究。验证实验后,进行了放大富集,研究了所开发策略在工业规模应用的可行性:结果:在溶液 pH 值为 2.0 时,XDA1 是制备 EGBL 的最合适吸附剂。此外,根据所需质量属性的限制条件,成功获得了操作参数的优化范围,验证实验证明了使用 DSD 研究制备 EGBL 的色谱过程的准确性和可靠性。最后,成功进行了放大实验,得到了 FG 含量为 26.54%、TT 含量为 8.96%、SA 含量为 10.70%的 EGBL,达到了国际标准 EGBL EGB761 的 SA 水平:本研究不仅为制备富含 SA 的 EGBL 提供了一种高效便捷的方法,而且加快了 GBL 高值化利用的步伐。
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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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