Survivin, β-catenin, and ki-67 immunohistochemical expression in canine perivascular wall tumors: Preliminary assessment of prognostic significance.

IF 2.3 2区 农林科学 Q2 PATHOLOGY Veterinary Pathology Pub Date : 2024-11-01 Epub Date: 2024-05-10 DOI:10.1177/03009858241246981
Francesco Godizzi, Federico Armando, Patrizia Boracchi, Giancarlo Avallone, Damiano Stefanello, Roberta Ferrari, Lavinia E Chiti, Andrea Cappelleri, Clarissa Zamboni, Silvia Dell'Aere, Attilio Corradi, Paola Roccabianca
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Abstract

High survivin expression has been correlated with poor outcomes in several canine tumors but not in soft tissue tumors (STTs). Survivin is a target gene of the Wnt/β-catenin pathway, which is involved in human STT oncogenesis. Immunohistochemistry for survivin, β-catenin, and Ki-67 was performed on 41 canine perivascular wall tumors (cPWTs), and statistical associations of protein expression and histopathologic and clinical variables with clinical outcomes were investigated. Immunohistochemically, there was nuclear positivity (0.9%-12.2% of tumor cells) for survivin in 41/41 (100%), cytoplasmic positivity (0 to > 75% of tumor cells) for survivin in 31/41 (76%), nuclear positivity (2.9%-67.2% of tumor cells) for β-catenin in 24/41 (59%), and cytoplasmic positivity (0% to > 75% of tumor cells) for β-catenin in 23/41 (56%) of cPWTs. All tumors expressed nuclear Ki-67 (2.2%-23.5%). In univariate analysis and multivariate analysis (UA and MA, respectively), every 1% increase of nuclear survivin was associated with an increase of the instantaneous death risk by a factor of 1.15 [hazard ratio (HR) = 1.15; P = .007]. Higher nuclear survivin was associated with grade II/III neoplasms (P = .043). Expression of cytoplasmic survivin, nuclear and cytoplasmic β-catenin, and nuclear Ki-67 were not significantly associated with prognosis in UA nor MA. Tumor size was a significant prognostic factor for local recurrence in UA [subdistribution HR (SDHR) = 1.19; P = .02] and for reduced overall survival time in MA. According to UA and MA, a unitary increase of mitotic count was associated with an increase of the instantaneous death risk by a factor of 1.05 (HR = 1.05; P = .014). Nuclear survivin, mitotic count, and tumor size seem to be potential prognostic factors for cPWTs. In addition, survivin and β-catenin may represent promising therapeutic targets for cPWTs.

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犬血管壁周围肿瘤中 Survivin、β-catenin 和 ki-67 的免疫组化表达:预后意义的初步评估。
存活素的高表达与几种犬肿瘤的不良预后有关,但与软组织肿瘤(STT)的不良预后无关。存活素是 Wnt/β-catenin 通路的一个靶基因,而 Wnt/β-catenin 通路参与了人类 STT 的致癌过程。研究人员对41例犬血管壁周肿瘤(cPWTs)进行了存活素、β-catenin和Ki-67的免疫组化,并对蛋白表达、组织病理学和临床变量与临床结果的统计学关联进行了研究。免疫组化结果显示,41/41(100%)的存活素呈核阳性(占肿瘤细胞的 0.9%-12.2%),31/41(76%)的存活素呈细胞质阳性(占肿瘤细胞的 0% 至 > 75%),核阳性(占肿瘤细胞的 2.在24/41(59%)的cPWTs中,β-catenin呈核阳性(占肿瘤细胞的2.9%-67.2%);在23/41(56%)的cPWTs中,β-catenin呈胞质阳性(占肿瘤细胞的0%->75%)。所有肿瘤均表达核 Ki-67(2.2%-23.5%)。在单变量分析和多变量分析(分别为 UA 和 MA)中,核存活素每增加 1%,瞬时死亡风险就会增加 1.15 倍[危险比 (HR) = 1.15;P = .007]。较高的核存活素与 II/III 级肿瘤相关(P = .043)。细胞质存活素、细胞核和细胞质β-catenin以及细胞核Ki-67的表达与UA和MA的预后无显著相关性。肿瘤大小是UA局部复发的重要预后因素[亚分布HR (SDHR) = 1.19; P = .02],也是MA总生存时间缩短的重要预后因素。根据 UA 和 MA,有丝分裂计数的单位增加与瞬时死亡风险增加 1.05 倍相关(HR = 1.05;P = .014)。核存活素、有丝分裂计数和肿瘤大小似乎是cPWT的潜在预后因素。此外,存活素和β-catenin可能是cPWTs有希望的治疗靶点。
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来源期刊
Veterinary Pathology
Veterinary Pathology 农林科学-病理学
CiteScore
4.70
自引率
8.30%
发文量
99
审稿时长
2 months
期刊介绍: Veterinary Pathology (VET) is the premier international publication of basic and applied research involving domestic, laboratory, wildlife, marine and zoo animals, and poultry. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of diseases of animals; reports experimental studies on mechanisms of specific processes; provides unique insights into animal models of human disease; and presents studies on environmental and pharmaceutical hazards.
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