{"title":"Quercetin promotes the proportion and maturation of NK cells by binding to MYH9 and improves cognitive functions in aged mice.","authors":"Tingting Su, Haitao Shen, Mengyuan He, Shanshan Yang, Xue Gong, Ce Huang, Liuling Guo, Hao Wang, Shengyu Feng, Taotao Mi, Meili Zhao, Qing Liu, Fengjiao Huo, Jian-Kang Zhu, Jianbo Zhu, Hongbin Li, Hailiang Liu","doi":"10.1186/s12979-024-00436-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonol compound widely distributed in plants that possesses diverse biological properties, including antioxidative, anti-inflammatory, anticancer, neuroprotective and senescent cell-clearing activities. It has been shown to effectively alleviate neurodegenerative diseases and enhance cognitive functions in various models. The immune system has been implicated in the regulation of brain function and cognitive abilities. However, it remains unclear whether quercetin enhances cognitive functions by interacting with the immune system.</p><p><strong>Results: </strong>In this study, middle-aged female mice were administered quercetin via tail vein injection. Quercetin increased the proportion of NK cells, without affecting T or B cells, and improved cognitive performance. Depletion of NK cells significantly reduces cognitive ability in mice. RNA-seq analysis revealed that quercetin modulated the RNA profile of hippocampal tissues in aging animals towards a more youthful state. In vitro, quercetin significantly inhibited the differentiation of Lin<sup>-</sup>CD117<sup>+</sup> hematopoietic stem cells into NK cells. Furthermore, quercetin promoted the proportion and maturation of NK cells by binding to the MYH9 protein.</p><p><strong>Conclusions: </strong>In summary, our findings suggest that quercetin promotes the proportion and maturation of NK cells by binding to the MYH9 protein, thereby improving cognitive performance in middle-aged mice.</p>","PeriodicalId":51289,"journal":{"name":"Immunity & Ageing","volume":null,"pages":null},"PeriodicalIF":5.2000,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11084035/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunity & Ageing","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12979-024-00436-1","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GERIATRICS & GERONTOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Quercetin is a flavonol compound widely distributed in plants that possesses diverse biological properties, including antioxidative, anti-inflammatory, anticancer, neuroprotective and senescent cell-clearing activities. It has been shown to effectively alleviate neurodegenerative diseases and enhance cognitive functions in various models. The immune system has been implicated in the regulation of brain function and cognitive abilities. However, it remains unclear whether quercetin enhances cognitive functions by interacting with the immune system.
Results: In this study, middle-aged female mice were administered quercetin via tail vein injection. Quercetin increased the proportion of NK cells, without affecting T or B cells, and improved cognitive performance. Depletion of NK cells significantly reduces cognitive ability in mice. RNA-seq analysis revealed that quercetin modulated the RNA profile of hippocampal tissues in aging animals towards a more youthful state. In vitro, quercetin significantly inhibited the differentiation of Lin-CD117+ hematopoietic stem cells into NK cells. Furthermore, quercetin promoted the proportion and maturation of NK cells by binding to the MYH9 protein.
Conclusions: In summary, our findings suggest that quercetin promotes the proportion and maturation of NK cells by binding to the MYH9 protein, thereby improving cognitive performance in middle-aged mice.
背景:槲皮素是一种广泛分布于植物中的黄酮醇化合物,具有多种生物特性,包括抗氧化、抗炎、抗癌、神经保护和清除衰老细胞等活性。研究表明,它能有效缓解神经退行性疾病,并在各种模型中增强认知功能。免疫系统与大脑功能和认知能力的调节有关。然而,槲皮素是否能通过与免疫系统相互作用来增强认知功能,目前仍不清楚:在这项研究中,中年雌性小鼠通过尾静脉注射槲皮素。槲皮素增加了 NK 细胞的比例,但不影响 T 细胞或 B 细胞,并改善了认知能力。消耗NK细胞会大大降低小鼠的认知能力。RNA-seq分析表明,槲皮素能调节衰老动物海马组织的RNA谱,使其趋于年轻化。在体外,槲皮素明显抑制了Lin-CD117+造血干细胞向NK细胞的分化。此外,槲皮素还能通过与MYH9蛋白结合促进NK细胞的比例和成熟:综上所述,我们的研究结果表明,槲皮素可通过与MYH9蛋白结合促进NK细胞的比例和成熟,从而改善中年小鼠的认知能力。
期刊介绍:
Immunity & Ageing is a specialist open access journal that was first published in 2004. The journal focuses on the impact of ageing on immune systems, the influence of aged immune systems on organismal well-being and longevity, age-associated diseases with immune etiology, and potential immune interventions to increase health span. All articles published in Immunity & Ageing are indexed in the following databases: Biological Abstracts, BIOSIS, CAS, Citebase, DOAJ, Embase, Google Scholar, Journal Citation Reports/Science Edition, OAIster, PubMed, PubMed Central, Science Citation Index Expanded, SCImago, Scopus, SOCOLAR, and Zetoc.