Anti-inflammatory Effects of Bacteroidota Strains Derived From Outstanding Donors of Fecal Microbiota Transplantation for the Treatment of Ulcerative Colitis.

IF 4.5 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Inflammatory Bowel Diseases Pub Date : 2024-11-04 DOI:10.1093/ibd/izae080
Dai Ishikawa, Xiaochen Zhang, Kei Nomura, Tomoyoshi Shibuya, Mariko Hojo, Makoto Yamashita, Satoshi Koizumi, Fuhito Yamazaki, Susumu Iwamoto, Masato Saito, Keisuke Kunigo, Ryosuke Nakano, Nakayuki Honma, Itaru Urakawa, Akihito Nagahara
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Abstract

Background: The proportion of certain Bacteroidota species decreased in patients with ulcerative colitis, and the recovery of Bacteroidota is associated with the efficacy of fecal microbiota transplantation therapy. We hypothesized that certain Bacteroidota may advance ulcerative colitis treatment. Accordingly, we aimed to evaluate the anti-inflammatory effects of Bacteroidota strains isolated from donors.

Methods: Donors with proven efficacy of fecal microbiota transplantation for ulcerative colitis were selected, and Bacteroidota strains were isolated from their stools. The immune function of Bacteroidota isolates was evaluated through in vitro and in vivo studies.

Results: Twenty-four Bacteroidota strains were isolated and identified. Using an in vitro interleukin (IL)-10 induction assay, we identified 4 Bacteroidota strains with remarkable IL-10-induction activity. Of these, an Alistipes putredinis strain exhibited anti-inflammatory effects in a mouse model of colitis induced by sodium dextran sulfate and oxazolone. However, 16S rRNA gene-based sequencing analysis of A. putredinis cultures in the in vivo study revealed unexpected Veillonella strain contamination. A second in vitro study confirmed that the coculture exhibited an even more potent IL-10-inducing activity. Furthermore, the production of A. putredinis-induced IL-10 was likely mediated via toll-like receptor 2 signaling.

Conclusions: This study demonstrated that A. putredinis, a representative Bacteroidota species, exhibits anti-inflammatory effects in vivo and in vitro; however, the effects of other Bacteroidota species remain unexplored. Our fecal microbiota transplantation-based reverse translation approach using promising bacterial species may represent a breakthrough in microbiome drug development for controlling dysbiosis during ulcerative colitis.

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从粪便微生物群移植治疗溃疡性结肠炎的优秀捐赠者中提取的类菌株的抗炎作用
背景:溃疡性结肠炎患者体内某些类杆菌的比例下降,而类杆菌的恢复与粪便微生物群移植疗法的疗效有关。我们推测,某些类杆菌可能会促进溃疡性结肠炎的治疗。因此,我们旨在评估从供体中分离出的类菌群菌株的抗炎作用:方法:筛选出粪便微生物群移植治疗溃疡性结肠炎疗效确切的供体,并从其粪便中分离出类杆菌属菌株。通过体外和体内研究评估了类杆菌的免疫功能:结果:共分离并鉴定出 24 株类杆菌。通过体外白细胞介素(IL)-10诱导试验,我们确定了 4 种具有显著 IL-10 诱导活性的类杆菌属菌株。其中,Alistipes putredinis菌株在右旋糖酐硫酸钠和恶唑酮诱导的小鼠结肠炎模型中表现出抗炎作用。然而,在体内研究中对 A. putredinis 培养物进行的基于 16S rRNA 基因的测序分析发现了意想不到的 Veillonella 菌株污染。第二次体外研究证实,这种共培养物具有更强的 IL-10 诱导活性。此外,A. putredinis 诱导的 IL-10 的产生可能是通过收费样受体 2 信号传导介导的:结论:本研究表明,A. putredinis(一种具有代表性的类杆菌属物种)在体内和体外都具有抗炎作用;然而,其他类杆菌属物种的作用仍有待探索。我们基于粪便微生物群移植的反向转化方法使用了有前景的细菌物种,这可能是微生物群药物开发的一个突破,可用于控制溃疡性结肠炎期间的菌群失调。
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来源期刊
Inflammatory Bowel Diseases
Inflammatory Bowel Diseases 医学-胃肠肝病学
CiteScore
9.70
自引率
6.10%
发文量
462
审稿时长
1 months
期刊介绍: Inflammatory Bowel Diseases® supports the mission of the Crohn''s & Colitis Foundation by bringing the most impactful and cutting edge clinical topics and research findings related to inflammatory bowel diseases to clinicians and researchers working in IBD and related fields. The Journal is committed to publishing on innovative topics that influence the future of clinical care, treatment, and research.
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