Marine bacteria have multiple polyamide 4-degrading enzymes.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-11-06 Epub Date: 2024-05-13 DOI:10.2323/jgam.2024.05.001
Yusuke Saito, Ibuki Jin, Miwa Yamada
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Abstract

Polyamide 4 (PA4) is expected to solve the issue of marine plastic pollution due to its excellent mechanical properties and biodegradability. In this study, to reveal the mechanism of PA4 biodegradation in the marine environment, we isolated 5 strains of PA4-degrading bacteria belonging to Aliiglaciecola, Dasania, and Pseudophaeobacter from a marine environment. The isolated 5 strains are novel PA4-degrading bacteria that are phylogenetically distinct from those isolated in previous studies. In addition, we compared the PA4-degrading activities and structures of the PA4-degrading enzymes secreted by the 5 strains and PA4-degrading strains isolated in our previous study. The PA4-degrading activity in the supernatant of the cultivation solutions differed among the strains. Native-PAGE and zymography using a polyacrylamide gel containing a PA4 emulsion demonstrated that PA4-degrading enzymes are classified into no less than three types of structures. These results suggested that marine PA4-degrading bacteria have multiple PA4-degrading enzymes. Our findings will contribute to a better understanding of the microbial degradation of PA4 in the marine environment.

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海洋细菌具有多种聚酰胺 4 降解酶。
聚酰胺 4(PA4)具有优异的机械性能和生物降解性,有望解决海洋塑料污染问题。在本研究中,为了揭示 PA4 在海洋环境中的生物降解机制,我们从海洋环境中分离出了 5 株 PA4 降解细菌,分别属于 Aliiglaciecola、Dasania 和 Pseudophaeobacter。这 5 株菌株是新型的 PA4 降解菌,在系统发育上与以往研究中分离到的细菌不同。此外,我们还比较了这 5 株菌株和之前研究中分离到的 PA4 降解菌株的 PA4 降解活性和分泌的 PA4 降解酶的结构。不同菌株培养液上清液中的 PA4 降解活性存在差异。使用含有 PA4 乳液的聚丙烯酰胺凝胶进行原生聚丙烯酰胺凝胶电泳和酶谱分析表明,PA4 降解酶可分为不少于三种类型的结构。这些结果表明,海洋 PA4 降解细菌具有多种 PA4 降解酶。我们的研究结果将有助于更好地了解海洋环境中 PA4 的微生物降解过程。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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