DENV-2 Outbreak Associated With Cosmopolitan Genotype Emergence in Western Brazilian Amazon.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS Bioinformatics and Biology Insights Pub Date : 2024-05-11 eCollection Date: 2024-01-01 DOI:10.1177/11779322241251581

Jessiane Rodrigues Ribeiro, Tárcio Peixoto Roca, Gleense Dos Santos Cartonilho, Ana Maísa Passos-Silva, Hillquias Monteiro Moreira, Karolaine Santos Teixeira, André Luiz Ferreira da Silva, Celina Aparecida Bertoni Lugtenburg, Alcione Oliveira Dos Santos, Juan Miguel Villalobos Salcedo, Valdinete Alves do Nascimento, Victor Costa de Souza, Rosemary Aparecida Roque, Marco Aurélio Krieger, Felipe Gomes Naveca, Rita de Cássia Pontello Rampazzo, Deusilene Souza Vieira

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Abstract

Background: Dengue virus (DENV) causes an important disease and directly affects public health, being the arbovirus that presents the highest number of infections and deaths in the Western Brazilian Amazon. This virus is divided into 4 serotypes that have already circulated in the region.

Methodology: Molecular characterization of a cohort containing 841 samples collected from febrile patients between 2021 and 2023 was analyzed using a commercial kit to detect the main arboviruses circulating in Brazil: Zika, DENV-1, DENV-2, DENV-3, DENV-4 and, Chikungunya. Subsequently, Sanger sequencing was performed for positive samples.

Results: The cohort detected 162 positive samples, 12 for DENV-1 and 150 identified as DENV-2, indicating co-circulation of serotypes. The samples were subjected to sequencing and the analysis of the sequences that obtained good quality revealed that 5 samples belonged to the V genotype of DENV-1 and 46 were characterized as DENV-2 Cosmopolitan genotype-lineage 5.

Conclusion: The results allowed us to identify for the first time the Cosmopolitan genotype in Rondônia, Brazilian Western Amazon, and its fast spread dispersion.

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巴西亚马孙河流域西部与世界性基因型出现有关的 DENV-2 爆发。

背景：登革热病毒（DENV）是巴西亚马孙河流域西部感染和死亡人数最多的虫媒病毒，是一种重要的疾病，直接影响公共卫生。该病毒分为 4 个血清型，已在该地区流行：使用商业试剂盒对 2021 年至 2023 年间从发热患者处收集的 841 份样本进行了分子特征分析，以检测在巴西流行的主要虫媒病毒：寨卡、DENV-1、DENV-2、DENV-3、DENV-4 和基孔肯雅。随后，对阳性样本进行了桑格测序：结果：研究组共检测到 162 份阳性样本，其中 12 份为 DENV-1，150 份为 DENV-2，这表明血清型是共同流行的。对样本进行了测序，并对获得的高质量序列进行了分析，发现5个样本属于DENV-1的V基因型，46个样本被鉴定为DENV-2 Cosmopolitan基因型-lineage 5：这些结果使我们首次确定了巴西亚马孙河流域西部朗多尼亚（Rondônia）的Cosmopolitan基因型，并确定了该基因型的快速扩散性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioinformatics and Biology Insights BIOCHEMICAL RESEARCH METHODS-

CiteScore

6.80

自引率

1.70%

发文量

审稿时长

8 weeks

期刊介绍： Bioinformatics and Biology Insights is an open access, peer-reviewed journal that considers articles on bioinformatics methods and their applications which must pertain to biological insights. All papers should be easily amenable to biologists and as such help bridge the gap between theories and applications.