Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Applied Oral Science Pub Date : 2024-05-10 eCollection Date: 2024-01-01 DOI:10.1590/1678-7757-2023-0294

Yun Qiu, Kailun Shen, Hongjiang Wei, Yufeng Zhang, Yulan Wang, Yi Bai

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Abstract

Objective: This study aims to develop a compound biomaterial to achieve effective soft tissue regeneration.

Methodology: Compound hyaluronic acid (CHA) and liquid horizontal-platelet-rich fibrin (H-PRF) were mixed at a ratio of 1:1 to form a CHA-PRF gel. Human gingival fibroblasts (HGFs) were used in this study. The effect of CHA, H-PRF, and the CHA-PRF gel on cell viability was evaluated by CCK-8 assays. Then, the effect of CHA, H-PRF, and the CHA-PRF gel on collagen formation and deposition was evaluated by qRT‒PCR and immunofluorescence analysis. Finally, qRT‒PCR, immunofluorescence analysis, Transwell assays, and scratch wound-healing assays were performed to determine how CHA, H-PRF, and the CHA-PRF gel affect the migration of HGFs.

Results: The combination of CHA and H-PRF shortened the coagulation time of liquid H-PRF. Compared to the pure CHA and H-PRF group, the CHA-PRF group exhibited the highest cell proliferation at all time points, as shown by the CCK-8 assay. Col1a and FAK were expressed at the highest levels in the CHA-PRF group, as shown by qRT‒PCR. CHA and PRF could stimulate collagen formation and HGF migration, as observed by fluorescence microscopy analysis of COL1 and F-actin and Transwell and scratch healing assays.

Conclusion: The CHA-PRF group exhibited greater potential to promote soft tissue regeneration by inducing cell proliferation, collagen synthesis, and migration in HGFs than the pure CHA or H-PRF group. CHA-PRF can serve as a great candidate for use alone or in combination with autografts in periodontal or peri-implant soft tissue regeneration.

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软组织再生的新方法：复合透明质酸和水平富血小板纤维蛋白组合的体外研究。

目的：本研究旨在开发一种复合生物材料，以实现有效的软组织再生：本研究旨在开发一种复合生物材料，以实现有效的软组织再生：将复合透明质酸（CHA）和液态水平血小板丰富纤维蛋白（H-PRF）以 1:1 的比例混合，形成 CHA-PRF 凝胶。本研究使用了人牙龈成纤维细胞（HGFs）。通过 CCK-8 试验评估了 CHA、H-PRF 和 CHA-PRF 凝胶对细胞活力的影响。然后，通过 qRT-PCR 和免疫荧光分析评估了 CHA、H-PRF 和 CHA-PRF 凝胶对胶原形成和沉积的影响。最后，进行了 qRT-PCR、免疫荧光分析、Transwell 试验和划痕伤口愈合试验，以确定 CHA、H-PRF 和 CHA-PRF 凝胶对 HGFs 迁移的影响：结果：CHA和H-PRF的组合缩短了液体H-PRF的凝结时间。与纯 CHA 和 H-PRF 组相比，CCK-8 检测显示 CHA-PRF 组在所有时间点的细胞增殖均最高。qRT-PCR 显示，Col1a 和 FAK 在 CHA-PRF 组的表达水平最高。通过荧光显微镜分析 COL1 和 F-肌动蛋白，以及 Transwell 和划痕愈合试验观察到，CHA 和 PRF 可刺激胶原蛋白的形成和 HGF 的迁移：结论：与纯 CHA 或 H-PRF 组相比，CHA-PRF 组通过诱导细胞增殖、胶原蛋白合成和 HGFs 迁移，在促进软组织再生方面表现出更大的潜力。在牙周或种植体周围软组织再生中，CHA-PRF 可单独使用或与自体移植物结合使用。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.