Effects of vitamin K2 administration on guided bone regeneration in diabetic rats

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontal research Pub Date : 2024-05-14 DOI:10.1111/jre.13287

Irmak Duman, Gamze Tanrıverdi, Hafize Öztürk Özener

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Abstract

Aim

The present study aimed to investigate the histomorphometric and immunohistochemical impacts of vitamin K2 on guided bone regeneration (GBR) in calvarial critical-size defects (CSDs) in diabetic rats.

Methods

A total of 30 rats were used in this study, comprising 12 non-diabetic (control) rats and 18 with streptozotocin-nicotinamide-induced experimental Diabetes mellitus (DM). In all rats, two calvarial CSDs were created: one defect was left empty (E), the other was treated with bovine-derived bone graft and collagen-based resorbable membrane (GM). Study groups were as follows: control rats administered saline (n = 6, C-E and C-GM groups) or vitamin K2 (n = 6, CK-E and CK-GM groups) and diabetic rats administered saline (n = 6, DM-E and DM-GM groups) or vitamin K2 (n = 6, DMK-E and DMK-GM groups). After 4 weeks of saline or vitamin K2 administration, the rats were euthanized. Bone defect healing and new bone formation were assessed histomorphometrically, and osteocalcin and osteopontin levels were examined immunohistochemically.

Results

Percentage of new bone formation was greater in CK-GM vs. CK-E and in DMK-GM vs. DMK-E [d = 3.86 (95% CI = 16.38–28.61), d = 1.86, (95% CI = 10.74–38.58), respectively, p < .05]. Bone defect healing scores were higher in CK-GM vs. CK-E and in DMK-GM vs. DMK-E [d = 2.69 (95% CI = -2.12 to −0.87), d = 3.28 (95% CI = 0.98–1.91), respectively, p < .05]. Osteocalcin expression levels were elevated in CK-GM vs. CK-E, in DMK-GM vs. DMK-E [d = 1.19 (95% CI = 0.08–1.41), d = 1.10 (95% CI = 0.02–1.22), respectively p < .05]. Vitamin K2 enhanced osteocalcin expression levels in DMK-E vs. DM-E [d = 2.78, (95% CI = 0.56–1.53), p < .05] and in DMK-GM vs. DM-GM [d = 2.43, (95% CI = 0.65–2.10), p < .05]. Osteopontin expression was enhanced in defects treated with GM vs. E defects [C-GM vs. C-E, d = 1.56 (95% CI = 0.38–2.01); CK-GM vs. CK-E, d = 1.91 (95% CI = 0.49–1.72); DM-GM vs. DM-E, d = 2.34 (95% CI = -1.12 to −0.50); DMK-GM vs. DMK-E, d = 2.00 (95% CI = 0.58–1.91), p < .05].

Conclusion

The research findings suggest that administering vitamin K2 in GBR for rats with DM favorably impacts bone healing in CSDs, presenting an adjunctive strategy for bone regeneration.

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服用维生素 K2 对糖尿病大鼠引导骨再生的影响

目的：本研究旨在探讨维生素 K2 对糖尿病大鼠腓骨临界大小缺损（CSD）中引导骨再生（GBR）的组织形态学和免疫组织化学影响：本研究共使用了 30 只大鼠，包括 12 只非糖尿病（对照组）大鼠和 18 只链脲佐菌素-烟酰胺诱导的实验性糖尿病（DM）大鼠。所有大鼠都有两个犊骨 CSD：一个缺损为空洞（E），另一个用牛源性骨移植和胶原基可吸收膜（GM）处理。研究分组如下：对照组大鼠服用生理盐水（n = 6，C-E 和 C-GM 组）或维生素 K2（n = 6，CK-E 和 CK-GM 组）；糖尿病大鼠服用生理盐水（n = 6，DM-E 和 DM-GM 组）或维生素 K2（n = 6，DMK-E 和 DMK-GM 组）。注射生理盐水或维生素 K2 4 周后，大鼠被安乐死。用组织形态计量学方法评估骨缺损愈合和新骨形成情况，用免疫组化方法检测骨钙素和骨素水平：结果：CK-GM 与 CK-E 相比，DMK-GM 与 DMK-E 相比，新骨形成的百分比更高[分别为 d = 3.86（95% CI = 16.38-28.61），d = 1.86，（95% CI = 10.74-38.58），p 结论：研究结果表明，在GBR中给DM大鼠服用维生素K2会对CSD的骨愈合产生有利影响，是骨再生的一种辅助策略。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.