Microsatellites for butterfly conservation: historical challenges, current relevance, and a guide to implementation

IF 2.4 3区环境科学与生态学 Q2 ECOLOGY Frontiers in Ecology and Evolution Pub Date : 2024-05-09 DOI:10.3389/fevo.2024.1344065

Emily Heffernan, Megan Barkdull, Noah Brady

引用次数: 0

Abstract

We are nearing the 20-year anniversary of a groundbreaking paper which details how microsatellite marker development in Lepidoptera is “extremely difficult for no apparent reason.” How far have we come in these past 20 years? Microsatellites are still the marker of choice in many population genetics studies for their ease of use, high degrees of polymorphism, species-specificity, and low cost. The rise of next-generation sequencing technologies (e.g. 454, Illumina, PacBio, etc.) has greatly advanced our abilities to generate many microsatellite markers per species. In this paper, we summarize the improvements in marker development using next-generation technology. Using case studies, we review the use and implementation of microsatellite markers in different conservation programs. Lastly, we provide a guide to data interpretation of microsatellite data generated for butterflies, with the goal of supporting student researchers and conservation practitioners in evaluating the meaning in their data.

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用于蝴蝶保护的微卫星：历史挑战、现实意义和实施指南

我们即将迎来一篇开创性论文发表 20 周年纪念，该论文详细描述了鳞翅目昆虫微卫星标记的开发是如何 "毫无理由地极其困难"。在过去的 20 年中，我们取得了哪些进展？微卫星因其易用性、高度多态性、物种特异性和低成本，仍然是许多群体遗传学研究的首选标记。新一代测序技术（如 454、Illumina、PacBio 等）的兴起大大提高了我们为每个物种生成大量微卫星标记的能力。在本文中，我们总结了利用下一代技术开发标记的进展。通过案例研究，我们回顾了微卫星标记在不同保护项目中的使用和实施情况。最后，我们提供了一份蝴蝶微卫星数据解读指南，旨在帮助学生研究人员和保护工作者评估数据的意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Ecology and Evolution Environmental Science-Ecology

CiteScore

4.00

自引率

6.70%

发文量

1143

审稿时长

12 weeks

期刊介绍： Frontiers in Ecology and Evolution publishes rigorously peer-reviewed research across fundamental and applied sciences, to provide ecological and evolutionary insights into our natural and anthropogenic world, and how it should best be managed. Field Chief Editor Mark A. Elgar at the University of Melbourne is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics and the public worldwide. Eminent biologist and theist Theodosius Dobzhansky’s astute observation that “Nothing in biology makes sense except in the light of evolution” has arguably even broader relevance now than when it was first penned in The American Biology Teacher in 1973. One could similarly argue that not much in evolution makes sense without recourse to ecological concepts: understanding diversity — from microbial adaptations to species assemblages — requires insights from both ecological and evolutionary disciplines. Nowadays, technological developments from other fields allow us to address unprecedented ecological and evolutionary questions of astonishing detail, impressive breadth and compelling inference. The specialty sections of Frontiers in Ecology and Evolution will publish, under a single platform, contemporary, rigorous research, reviews, opinions, and commentaries that cover the spectrum of ecological and evolutionary inquiry, both fundamental and applied. Articles are peer-reviewed according to the Frontiers review guidelines, which evaluate manuscripts on objective editorial criteria. Through this unique, Frontiers platform for open-access publishing and research networking, Frontiers in Ecology and Evolution aims to provide colleagues and the broader community with ecological and evolutionary insights into our natural and anthropogenic world, and how it might best be managed.