Design of Highly Porous Materials Based on Chitosan/Pectin Interpolyelectrolyte Complex for Wound Healing Application

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-05-09 DOI:10.1155/2024/8747902
Aliaksandr Kraskouski, Maksim Mashkin, Viktoryia Kulikouskaya, Viktoryia Savich, Anastasiya Sidarenka, Sergei Pinchuk, Ruibin Li
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Abstract

Interpolyelectrolyte complexes (IPECs) of polysaccharides are multifunctional polymer materials that improve the mechanical and physicochemical properties of individual polysaccharides. In this study, highly porous (>90%) materials based on IPECs of versatile natural polysaccharides, chitosan (30 and 1,200 kDa) and pectin, are obtained by freeze-drying technique. To enhance the interaction between chitosan and pectin macromolecules, the latter are chemically functionalized with dialdehyde groups. The chitosan-/aldehyde-functionalized pectin (Chit/AF-Pect) polyelectrolyte complex sponges obtained are characterized using SEM, FTIR spectroscopy, and TGA. The swelling capacity study reveals a higher swelling ratio of IPEC sponges with an increase in both the molecular weight and content of chitosan: for Chit30/AF-Pect, the swelling ratio rises from 327% to 480%, while for Chit1200/AF-Pect, from 681% to 1,066%. Additionally, the in vitro degradation test demonstrates higher stability of Chit1200/AF-Pect sponges in comparison with those of Chit30/AF-Pect: after 4 days of incubation, the weight losses are found to be 9%–16% and 18%–41%, respectively. The cytotoxicity study shows that Chit30/AF-Pect sponges are noncytotoxic, with cell viability values >70%. Furthermore, the Chit30/AF-Pect sponges, obtained at chitosan:pectin weight ratio of 5:1, exhibit bactericidal activity against Escherichia coli BIM B-984 G, Pseudomonas aeruginosa BIM B-807 G, Staphylococcus aureus BIM B-1841, and slightly inhibit the growth of Enterococcus faecalis BIM B-1530 G. These findings indicate that the obtained Chit30/AF-Pect sponges can be used to create wound dressings for wound healing applications.

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设计基于壳聚糖/果胶间聚电解质复合物的高多孔材料,用于伤口愈合应用
多糖间电解质复合物(IPECs)是一种多功能聚合物材料,可改善单个多糖的机械和理化特性。本研究通过冷冻干燥技术获得了基于多功能天然多糖--壳聚糖(30 和 1,200 kDa)和果胶--的 IPECs 的高孔隙率(90%)材料。为了增强壳聚糖和果胶大分子之间的相互作用,果胶大分子被二醛基团化学官能化。利用扫描电镜、傅立叶变换红外光谱和热重分析法对获得的壳聚糖/甲醛官能化果胶(Chit/AF-Pect)聚电解质复合物海绵进行了表征。溶胀能力研究表明,随着壳聚糖分子量和含量的增加,IPEC 海绵的溶胀率也会增加:Chit30/AF-Pect 的溶胀率从 327% 增加到 480%,而 Chit1200/AF-Pect 的溶胀率则从 681% 增加到 1066%。此外,体外降解测试表明,与 Chit30/AF-Pect 相比,Chit1200/AF-Pect 海绵具有更高的稳定性:培养 4 天后,重量损失分别为 9%-16% 和 18%-41%。细胞毒性研究表明,Chit30/AF-Pect 海绵无细胞毒性,细胞存活率为 70%。此外,壳聚糖与果胶重量比为 5:1 的 Chit30/AF-Pect 海绵对大肠杆菌 BIM B-984 G、铜绿假单胞菌 BIM B-807 G 和金黄色葡萄球菌 BIM B-1841 具有杀菌活性,并能轻微抑制粪肠球菌 BIM B-1530 G 的生长。这些研究结果表明,获得的 Chit30/AF-Pect 海绵可用于制作伤口愈合用敷料。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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