Chlorophyll a fluorescence and phytochemical response in blackberry species ( Rubus sp) cultivated in the high tropics

IF 1.5 4区农林科学 Q3 PLANT SCIENCES Journal of Berry Research Pub Date : 2024-05-09 DOI:10.3233/jbr-230043

Brigitte Liliana Moreno-Medina, Fánor Casierra-Posada, Miguel Ángel García-Parra

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Abstract

BACKGROUND: The evaluation of chlorophyll fluorescence activity is a useful tool for rapid and effective assessments of the effect of abiotic factors on the physiology of different crops. In Rubus, this type of measurements describes tolerance to high temperatures, sensitivity to heat and morphological alterations in flowering and chlorophyll content, as a result of plant stress. OBJECTIVE: The present research estimated chlorophyll fluorescence and the presence of some phenolic compounds in the leaves of three cultivars of blackberry (Rubus sp.) established in the central region of Colombia, under conditions of open air between 2400 and 2800 masl. MATERIAL AND METHODS: This was done through the measurement of chlorophyll a (Junior-PAM II modulated fluorometer), the quantification of phenolic compounds (Folin Ciocalteu), the determination of antioxidant capacity (DPPH and ABTS) and the study of phenolic profiles (UHPLC) in leaves of three blackberry cultivars. RESULTS: Cultivars R. alutaceus (0.66), R. glaucus (0.73) and R. alpinus (0.76), presented values lower than the optimum (0.83), for the parameter F v /F m . In addition, the direct relationships between the parameters that define the behavior of PSII and the accumulation of different polyphenols were described. Reference was also made to the content of phenolic compounds (ranging from 125 to 150 mg GA g–1 dw), which did not show significant statistical differences. The heat map and principal component analysis show the close relationship between the parameters that characterize the functioning of PSII and the accumulation of phenolic compounds, as a mechanism of adaptation to the adverse conditions of the blackberry cultivars studied. CONCLUSIONS: The results reveal that the leaves of the Rubus studied could be a source of functional ingredients for use in the cosmetic, nutraceutical and food industries and epigallocatechin was attributed the greatest responsibility for the antioxidant capacity.

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高热带地区栽培的黑莓品种（Rubus sp）的叶绿素 a 荧光和植物化学反应

背景：叶绿素荧光活性评估是一种有用的工具，可用于快速有效地评估非生物因素对不同作物生理机能的影响。在鲁冰花（Rubus）中，这种测量方法描述了对高温的耐受性、对热的敏感性以及植物胁迫导致的开花和叶绿素含量的形态变化。目的：本研究估测了哥伦比亚中部地区三个黑莓（Rubus sp.）栽培品种在 2400 至 2800 米高的露天条件下叶片的叶绿素荧光和一些酚类化合物的含量。材料与方法：通过测量叶绿素 a（Junior-PAM II 调制荧光计）、酚类化合物定量（Folin Ciocalteu）、抗氧化能力测定（DPPH 和 ABTS）和酚类概况研究（超高效液相色谱法）来测定三种黑莓栽培品种叶片中的酚类化合物。结果：R. alutaceus（0.66）、R. glaucus（0.73）和 R. alpinus（0.76）的参数 F v/F m 值低于最佳值（0.83）。还参考了酚类化合物的含量（125 至 150 毫克 GA g-1 干重），其统计差异并不显著。热图和主成分分析表明，表征 PSII 功能的参数与酚类化合物的积累之间关系密切，这是黑莓栽培品种适应不利条件的一种机制。结论：研究结果表明，所研究的茜草叶可作为化妆品、营养保健品和食品行业使用的功能性成分的来源，表没食子儿茶素在抗氧化能力方面负有最大责任。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.