A lysing polysaccharide monooxygenase from Aspergillus niger effectively facilitated rumen microbial fermentation of rice straw.

IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Bioscience Pub Date : 2024-10-01 Epub Date: 2024-05-07 DOI:10.5713/ab.24.0026

Ting Li, Kehui OuYang, Qinghua Qiu, Xianghui Zhao, Chanjuan Liu

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Abstract

Objective: This study investigated the impact of Aspergillus niger lysing polysaccharide monooxygenase (AnLPMO) on in vitro rumen microbial fermentation of rice straw.

Methods: AnLPMO was heterologously expressed in Escherichia coli. Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy analyzed the surface structure of rice straw after AnLPMO treatment. Two in vitro experiments, coupled with 16S highthroughput sequencing and quantitative real-time polymerase chain reaction techniques, assessed the influence of AnLPMO on rumen microbial fermentation of rice straw.

Results: AnLPMO exhibited peak activity at 40°C and pH 6.5, with a preference for rice straw xylan hydrolysis, followed by Avicel. AnLPMO application led to the fractional removal of cellulose and hemicelluloses and a notable reduction in the levels of carbon elements and C-C groups present on the surface of rice straw. Compared to the control (no AnLPMO), supplementing AnLPMO at 1.1 to 2.0 U significantly enhanced in vitro digestibility of dry matter (IVDMD, p<0.01), total gas production (p<0.01), and concentrations of total volatile fatty acids (VFA, p<0.01), acetate (p<0.01), and ammonia-N (p<0.01). Particularly, the 1.4 U AnLPMO group showed a 14.8% increase in IVDMD. In the second experiment, compared to deactivated AnLPMO (1.4 U), supplementing bioactive AnLPMO at 1.4 U increased IVDMD (p = 0.01), total gas production (p = 0.04), and concentrations of total VFA (p<0.01), propionate (p<0.01), and ammonia-N (p<0.01), with a limited 9.6% increase in IVDMD. Supplementing AnLPMO stimulated the growth of ruminal bacterial taxa facilitating fiber degradation, including Proteobacteria, Spirochaetes, Succinivibrio, Rikenellaceae_RC9_ Gut_Group, Prevotelaceae_UCG-003, Desulfovibrio, Fibrobacter succinogenes, Ruminococcus albus, R. flavefaciens, Prevotella bryantii, P. ruminicola, and Treponema bryantii.

Conclusion: These findings highlight AnLPMO's potential as a feed additive for improving rice straw utilization in ruminant production.

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黑曲霉的裂解多糖单加氧酶有效促进了稻草的瘤胃微生物发酵。

研究目的本研究探讨了黑曲霉裂解多糖单加氧酶（AnLPMO）对水稻秸秆体外瘤胃微生物发酵的影响：方法：在大肠杆菌中异源表达 AnLPMO。傅立叶变换红外光谱法和 X 射线光电子能谱分析了 AnLPMO 处理后稻草的表面结构。两个体外实验结合 16S 高通量测序和 qRT-PCR 技术，评估了 AnLPMO 对稻草瘤胃微生物发酵的影响：结果：AnLPMO 在 40 °C、pH 值为 6.5 时表现出峰值活性，优先水解稻草木聚糖，其次是 Avicel。使用 AnLPMO 可去除部分纤维素和半纤维素，并显著降低稻草表面碳元素和 C-C 基团的含量。与对照组（不添加 AnLPMO）相比，添加 1.1 U-2.0 U 的 AnLPMO 可显著提高干物质的体外消化率（IVDMD，P < 0.01）、总产气量（P < 0.01）以及总挥发性脂肪酸（VFA，P < 0.01）、醋酸盐（P < 0.01）和氨氮（P < 0.01）的浓度。特别是，1.4 U AnLPMO 组的 IVDMD 增加了 14.8%。在第二个实验中，与失活的 AnLPMO（1.4 U）相比，补充 1.4 U 的生物活性 AnLPMO 可增加 IVDMD（P = 0.01）、总产气量（P = 0.04）以及总 VFA（P < 0.01）、丙酸盐（P < 0.01）和氨-N（P < 0.01）的浓度，但 IVDMD 仅增加了 9.6%。补充 AnLPMO 可刺激促进纤维降解的瘤胃细菌类群的生长，包括蛋白细菌、螺旋体、琥珀弧菌、Rikenellaceae_RC9_Gut_Group、Prevotelaceae_UCG-003、脱硫弧菌、琥珀酸纤维菌、白反刍球菌、R. flavefaciens、布氏前驱菌、反刍螺菌和布氏特雷庞氏菌：这些发现凸显了 AnLPMO 作为饲料添加剂在提高反刍动物生产中稻草利用率方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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