Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation.

IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animal Bioscience Pub Date : 2024-09-01 Epub Date: 2024-05-07 DOI:10.5713/ab.24.0030

Silong Zhang, Hanbing Zhang, Kexiong Liu, Xiaoling Xu, Yusheng Qin, Linli Xiao, Chunmei Zhou, Jianliang Wu, Yan Liu, Jiahua Bai

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Abstract

Objective: This study investigated the efficacy of different concentrations of cholesterolloaded cyclodextrin (CLC) on cryopreservation in boar sperm quality.

Methods: In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP), and structural- and oxidative-damage related proteins in all groups after thawing.

Results: The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p<0.05). The plasma membrane integrity rate, acrosome integrity rate, and total motility rate of sperm were also enhanced after thawing in the CLC-treated group (all p<0.05). Moreover, ROS and MDA production and ATP loss were reduced in CLC-treated sperm during freezing and thawing (p<0.05). Finally, CLC pretreatment partially prevented the consumption of various proteins involved in metabolism including capping actin protein of muscle Z-line subunit beta (CAPZB), heat shock protein 90 alpha family class A member 1 (HSP90AA1) and phosphoglycerate mutase 2 (PGAM2) (p<0.05).

Conclusion: The CLC treatment increased cholesterol concentration and decreased structural injury and oxidative damage during boar sperm freezing and thawing, improving the efficacy of sperm cryopreservation in boar.

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胆固醇环糊精处理对公猪精子冷冻的影响

研究目的本研究探讨了不同浓度的胆固醇环糊精（CLC）对冷冻保存公猪精子质量的影响：本研究在冷冻前用不同浓度的环糊精处理公猪精子，分析冻融前后精子胆固醇浓度、质膜、顶体完整率和总运动率。我们还研究了解冻后各组精子中活性氧（ROS）、丙二醛（MDA）、ATP、结构和氧化损伤相关蛋白的水平：结果显示，冷冻前和解冻后，CLC 处理组的胆固醇浓度均高于对照组（P < 0.05）。解冻后，CLC 处理组精子的质膜完整率、顶体完整率和总运动率也有所提高（均 p < 0.05）。此外，经 CLC 处理的精子在冷冻和解冻过程中减少了 ROS 和 MDA 的产生以及 ATP 的损失（P < 0.05）。最后，CLC预处理部分阻止了参与新陈代谢的各种蛋白质的消耗，包括CAPZB、HSP90AA1和PGAM2（p < 0.05）：结论：CLC 处理提高了胆固醇浓度，减少了公猪精子冷冻和解冻过程中的结构损伤和氧化损伤，提高了公猪精子冷冻保存的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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