{"title":"Towards a portable setup for the on-site SERS detection of miRNAs","authors":"Chiara Novara","doi":"10.1051/jeos/2024028","DOIUrl":null,"url":null,"abstract":"The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by Surface Enhanced Raman Scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the European Optical Society-Rapid Publications","FirstCategoryId":"4","ListUrlMain":"https://doi.org/10.1051/jeos/2024028","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by Surface Enhanced Raman Scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.
期刊介绍：
Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, security, sensing, bio- and medical sciences, healthcare and chemistry.
Recent achievements in other sciences have allowed continual discovery of new natural mysteries and formulation of challenging goals for optics that require further development of modern concepts and running fundamental research.
The Journal of the European Optical Society – Rapid Publications (JEOS:RP) aims to tackle all of the aforementioned points in the form of prompt, scientific, high-quality communications that report on the latest findings. It presents emerging technologies and outlining strategic goals in optics and photonics.
The journal covers both fundamental and applied topics, including but not limited to:
Classical and quantum optics
Light/matter interaction
Optical communication
Micro- and nanooptics
Nonlinear optical phenomena
Optical materials
Optical metrology
Optical spectroscopy
Colour research
Nano and metamaterials
Modern photonics technology
Optical engineering, design and instrumentation
Optical applications in bio-physics and medicine
Interdisciplinary fields using photonics, such as in energy, climate change and cultural heritage
The journal aims to provide readers with recent and important achievements in optics/photonics and, as its name suggests, it strives for the shortest possible publication time.
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