Decolorization of Crystal Violet by a Mixed Culture under the Influence of Bioelectrochemical Stimulation

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-05-16 DOI:10.1134/S0003683824603585

A. A. Samkov, E. V. Pankratova, M. N. Kruglova, A. V. Bespalov, S. M. Samkova, N. N. Volchenko, A. A. Khudokormov

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Abstract

A significant variation in the relative representation of copies of bacterial genes of dye-decolorizing DyP peroxidases typical for the genus Shewanella and a number of other microorganisms was found in the bottom sediments of freshwater reservoirs. It was found that the specific rate of decolorization of crystal violet in a laboratory bioelectrochemical system by a mixed culture of bottom sediments, which showed the highest representation of DyP genes, depended on the method of electrical stimulation of the external circuit and the concentration of the dye. After an increase in the concentration of more than 20 microns, the maximum speed was achieved in the presence of an ionistor polarly connected to the external electrical circuit of the bioelectrochemical system and amounted to 3.23 ± 0.11 μM/h, while with the opposite polarity connection, a minimum value of 2.07 ± 0.08 μM/h was observed. In the case of an open circuit and a resistor, similar indicators occurred with 2.88 ± 0.09 and 2.67 ± 0.12 μM/h, respectively. When analyzing the decolorization products, a consistent decrease in the maxima of the absorption bands of the dye was noted, indicating its more complete degradation by the mixed culture. The results may be of interest for the development of methods to improve the efficiency of bioelectrochemical methods of environmental biotechnology by electrostimulation of the external circuit.

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生物电化学刺激下混合培养物对水晶紫的脱色作用

在淡水水库的底层沉积物中发现，典型的雪旺氏菌属和其他一些微生物的染料脱色 DyP 过氧化酶细菌基因拷贝的相对代表性存在很大差异。研究发现，在实验室生物电化学系统中，底层沉积物混合培养物对水晶紫脱色的特定速率取决于外电路的电刺激方法和染料的浓度。在浓度增加到 20 微米以上后，与生物电化学系统外部电路极性连接的离子电阻器的速度达到最大，为 3.23 ± 0.11 μM/h，而反极性连接的离子电阻器的速度最小，为 2.07 ± 0.08 μM/h。在开路和电阻的情况下，也出现了类似的指标，分别为 2.88 ± 0.09 和 2.67 ± 0.12 μM/h。在分析脱色产物时，发现染料吸收带的最大值持续下降，表明混合培养物对染料的降解更为彻底。这些结果可能对开发通过电刺激外电路来提高环境生物技术中生物电化学方法效率的方法有意义。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.