Effects of CO2 and Soil Moisture Treatments on Morphological and Allometric Trait Variation in Coppiced Seedlings: A Study of Four Early-Successional Deciduous Species

Abstract

Atmospheric CO2 levels have been increasing, and likewise, increasing drought events have been following increasing temperatures. There is very little literature on the effects of climate change factors on early-successional deciduous species used for ecological restoration. Thus, morphological and allometric variation in four coppiced early-successional deciduous species was examined in response to a 2 × 2 factorial of ambient CO2 (aCO2, 400 ppm) and elevated CO2 (eCO2, 800 ppm), as well as well-watered and drought treatments with 15%–20% and 5%–10% volumetric moisture content, respectively, grown in sandy soil with low soil nitrogen (N) under greenhouse conditions. The four species examined were as follows: green alder (Alnus viridis subsp. crispa (Ait.) Turrill), speckled alder (A. incana subsp. rugosa (Du Roi) R.T. Clausen), gray birch (Betula populifolia (Marshall)), and white birch (B. papyrifera (Marshall)), and all are from the same phylogenetic family, Betulaceae. Genus differences in morphological and growth traits were large, especially in response to the environmental treatments used. Alders upregulated all growth traits under eCO2 because of the strong coppicing sink effect and the additional foliar N provided by the actinorhizal ability of the genus, whereas birches remained the same or slightly decreased under eCO2. As a result, alders have a significantly greater foliar N than birches, with 2.8 and 1.0%, respectively. All species reduced growth under drought, and green alder had the greatest stem dry mass growth, followed by speckled alder and then the birches. Under drought, eCO2 not only mitigated the alder drought dry mass but, in fact, doubled the stem dm, whereas eCO2 only just mitigated the birches drought response. When corrected for size using stem height, alders allocated more to stem and leaf and less to root dry mass than birches. Atmospheric CO2 and soil moisture treatments changed organ biomass allocation. The tallest stem height was the best predictor of total (above and below) dry mass. With increasing atmospheric CO2, particularly on low nutrient sites, the results show alders are capable of sequestering far more carbon than birches. In addition, with more atmospheric CO2, alders can mitigate against drought conditions better compared to birches.