Biomarker Evaluation in Toxoplasma gondii-infected Patients

Abstract

Back ground: This study used Toxoplasma IgG/IgM Antibody Rapid test to detect Toxoplasmosis in pregnant women and evaluate their lipid profile compared to a healthy control group. The aim of the study: Serodiagnosis of parasite infection in aborted pregnant women using the rapid detection method. Evaluating the level of some immunological parameters in infected women such as IL17-A, TNA-, lactoferrin, shedding light on the lipid profile of parasite-infected women. Patients and Methods: blood samples 240 were collected from aborted pregnant women attending some governmental hospitals and private medical clinics in some areas of Salah al-Din Governorate during the period from 1/7/2022 to 1/2/2023 and the required information was recorded according to a special questionnaire form. The results: were analyzed statistically using the IBM SPSS Statistics (version 23) program, according to the t-test, at a significant level of p≤0.05. The results of Rapid Test (93 positive samples) 38.7% (divided into 80) 86.02% (positive samples for IgG, while 13 sample) 13.97% (positive for IgM and 147 negative samples) 61.25%. (There is a positive correlation between (LTF, IL-17A, TNF-a) (highly significant). The lipid profile analysis revealed a non-significant increase for each of cholesterol and high-density lipoprotein (HDL) in infected women, as Mean ± S.E concentration was (168.4±8.2 Pg/ml); (33±1.5pg/ml) respectively, compared to the control group, where the Mean ± S.E concentration was (141.6±15.5 Pg/ml); (28±3.09Pg/ml) respectively. While there was a non-significant decrease in each of LDL, very low-density lipoprotein (VLDL), and triglycerides in pateints group, as Mean ± S.E concentration was (80.6±3.7pg/ml); (45.4±1.07 pg/ml); (159.7±4 pg/ml), respectively, compared to the control group, where the average concentration was (84.3±5.3pg/ml); (48.6±2pg/ml) 161.3±6.7pg/ml) respectively.