Whole genome sequence resources for Pseudomonas amygdali pv. sesami and Xanthomonas arboricola isolated from sesame (Sesamum indicum) in North Carolina in 2022

Abstract

Sesame (Sesamum indicum) is a specialty with increasing interest as an alternative, rotational crop for US agricultural systems. This crop is susceptible to several pathogens, including Pseudomonas and Xanthomonas species. Two bacterial isolates were recently cultured from sesame in North Carolina symptomatic of bacterial spot and identified as Pseudomonas amygdali pv. sesami (Pas) and Xanthomonas sp. These isolates were used for hybrid whole genome sequencing and assembly using Illumina and PacBio to develop robust genomic resources for these disease-causing strains. The results provide the most complete Pas genome available and placed the Xanthomoas sp. isolate into the X. arboricola species designation. Improved genomic resources for pathogens of sesame are needed to accurately detect, characterize, and employ timely management of the disease.