A simple, rapid, and green method for determination of three cholic acids in bio-transformed Jindanfen by HPLC-MS

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-05-08 DOI:10.1556/1326.2024.01209

Zhengming Qian, Qi Huang, Feng Wang, Qing-Qing Lei, Zhuobin He, Qilong Wu, Menghua Wu, Jin Gao

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Abstract

A simple, rapid, and green high-performance liquid chromatography-mass spectrometry (HPLC-MS) method was developed for determination of tauroursodeoxycholic acid (TUDCA), taurocholic acid (TCA), and taurochenodeoxycholic acid (TCDCA) in bio-transformed Jindanfen (BTJDF), which is obtained from chicken bile through a bioconversion process. The sample was prepared using water. The HPLC separation was operated on a poroshell 120 EC-C18 column with a 2.0 min gradient elution procedure. Detection was performed on a single quadrupole mass spectrometer in negative mode with selected ion monitoring mode (SIM). This developed HPLC-MS method presented good linearity (r > 0.997) and sensitivity (limit of quantification, 30.0–80.0 pg) for three analytes. The relative standard deviations (RSDs) for precision, repeatability, and stability were all below 3.00%. The matrix effects and average recoveries of three analytes were 91.2–97.9% (RSDs < 1.50%) and 95.4–103% (RSDs < 3.00%), respectively. The average contents of TUDCA, TCA, and TCDCA in ten batches of samples were 33.8, 13.2, and 20.5%, respectively. Finally, the greenness of the developed method was validated by Analytical Eco-Scale and Complex-GAPI. The developed method was proved to be an eco-friendly, effective, and reliable approach for assaying the three cholic acids in BTJDF, which is help to improve the quality evaluation level of the BTJDF industry.

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利用 HPLC-MS 测定生物转化金丹芬中三种胆酸的简单、快速、绿色方法

建立了一种简便、快速、绿色的高效液相色谱-质谱（HPLC-MS）测定金丹芬（BTJDF）中牛磺酸去氧胆酸（TUDCA）、牛磺酸去氧胆酸（TCA）和牛磺酸去氧胆酸（TCDCA）的方法。样品用水制备。高效液相色谱分离采用孔壳 120 EC-C18 色谱柱，梯度洗脱程序为 2.0 分钟。检测在单四极杆质谱仪上进行，采用负离子模式和选择离子监测模式（SIM）。所开发的 HPLC-MS 方法对三种分析物具有良好的线性（r > 0.997）和灵敏度（定量限为 30.0-80.0 pg）。精密度、重复性和稳定性的相对标准偏差（RSD）均低于 3.00%。三种分析物的基质效应和平均回收率分别为 91.2-97.9%（RSD < 1.50%）和 95.4-103%（RSD < 3.00%）。10批样品中TUDCA、TCA和TCDCA的平均含量分别为33.8%、13.2%和20.5%。最后，所建立的方法的绿色性得到了分析生态尺度和 Complex-GAPI 的验证。所建立的方法是一种环保、有效、可靠的检测BTJDF中三种胆酸的方法，有助于提高BTJDF行业的质量评价水平。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.