Effect of mangiferin isolated from Mangifera indica leaves on in vitro blood coagulation and cell migration activities

Abstract

Background: Mangiferin, a natural compound has been reported to possess a variety of biological activities such as anticancer, anti-diabetic, antimicrobial, antioxidant, cardioprotective activities, etc. Screening on blood coagulation activity effects of mangiferin might be helpful for further activities investigation. Objective: This study aimed to isolate mangiferin from mango leaves and evaluate its blood coagulation and anticancer activities against human lung cancer cell lines (A549 cells). Materials and methods: Mangiferin was extracted from mango leaves and characterized by IR, NMR, and mass spectroscopic techniques. It was determined in vitro activities as follows: blood clotting time, platelet aggregation, activated partial thromboplastin time (aPTT), prothrombin time (PT), clot lysis, fibrinolysis, and cell migration assay. Results: It was found that mangiferin had a significantly slower effect on inducing blood clots than the control group, with the coagulation value of 13.09±2.97 minutes and decreasing platelet aggregation at an inhibition percentage value of 14.1±1.2. There was significant (p<0.05) prolongation of PT and aPTT activities tested with the mangiferin at the value of 12.4±1.2 and 29.9±3.1 seconds, respectively. However, mangiferin was unable to cause fibrin clot dissolution on fibrinolysis test. Mangiferin also showed anticancer activity against A549 cells by inhibition of cell migration assay. Conclusion: Mangiferin showed antiplatelet aggregation activity and prolongation of prothrombin time (PT) and activated partial thromboplastin time (aPTT) assay without fibrinolysis activity. In addition, mangiferin showed anticancer activity against A549 cells by inhibiting cell migration.