Morphological and molecular diversity analysis vis-a-vis phylogenetic correlation among pomegranate (Punica granatum L.) varieties

Abstract

Identification of different pomegranate (Punica granatum L.) genotypes using morphological descriptors and further substantiated through molecular means is essential for a breeding program. Characterization using molecular markers has an advantage over its morphological counterpart due to its low expense, quick and reliable results, and the requirement of less sophisticated equipment. In this study, eight different pomegranate cultivars were characterized based on their morphological and molecular characters in sub-tropical regions between 2018 and 2019. Morphological experiments were conducted in a randomized block design replicated thrice for each cultivar, whereas RAPD markers were employed for molecular studies. Principal component analysis revealed significant morphological variation (79.61%) among the cultivars. A high level of polymorphism, similar to PCA, was observed with RAPD analysis (80.49%). Some discrepancies between morphological and molecular results were noted in UPGMA cluster analysis. However, eight primers (total = 41) distinctively separated the Bhagwa variety from others, with the maximum similarity coefficient value (ca. 0.54) and superior fruit quality distinguished in PCA analysis with the maximum mean Euclidean distance (ca. 16). Hence, RAPD markers could be used along with morphological markers for a quick screening of germplasms in further crop improvement programs.