Analysis of ginseng rusty root symptoms transcriptome and its pathogenesis directed by reactive oxygen species theory

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-05-01 DOI:10.1002/pld3.586

Pengcheng Yu, Xiaowen Song, Wei Zhang, Yao Yao, Junling Ren, Liyang Wang, Wenfei Liu, Zhaoping Meng, Xiangcai Meng

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Abstract

Abstract Ginseng rusty root symptoms (GRS) is a primary disease of ginseng, which seriously decreases the yield and quality of ginseng and causes enormous losses to ginseng production. GRS prevention and control is still challenging due to its unclear etiology. In this study, the phloem tissue of healthy Panax ginseng (AG), the nonred tissue of the phloem epidermis around the lesion (BG), and the red lesion site tissue of GRS (CG) were extracted for mRNA transcriptomic analysis; 35,958 differentially expressed genes (DEGs) were identified and were associated with multiple stress resistance pathways, reactive oxygen species (ROS), and iron ion binding. Further study showed that the contents of O2 •‐, H2O2, and malondialdehyde (MDA) were significantly increased in BG and CG tissues. Under anaerobic conditions caused by excessive soil moisture, the overproduction of ROS destroys cell membranes, simultaneously converting Fe2+ to Fe3+ and depositing it in the cell wall, which results in GRS, as evidenced by the success of the GRS induction test.

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人参锈根症状转录组分析及其由活性氧理论指导的致病机理

摘要人参锈根病（GRS）是人参的一种主要病害，严重降低人参的产量和质量，给人参生产造成巨大损失。由于病因不清，人参锈根症的防控仍面临挑战。本研究提取了健康人参的韧皮部组织（AG）、病变周围的非红色韧皮部表皮组织（BG）和GRS的红色病变部位组织（CG）进行mRNA转录组分析，发现了35958个差异表达基因（DEG），这些基因与多种抗应激途径、活性氧（ROS）和铁离子结合有关。进一步的研究表明，BG 和 CG 组织中的 O2-、H2O2 和丙二醛（MDA）含量显著增加。在土壤水分过多造成的厌氧条件下，ROS的过度产生会破坏细胞膜，同时将Fe2+转化为Fe3+并沉积在细胞壁中，从而导致GRS，GRS诱导试验的成功就证明了这一点。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.