Occurrence and characteristics of blaOXA-181-carrying Klebsiella aerogenes from swine in China

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-05-17 DOI:10.1016/j.jgar.2024.04.009
Lin Sun , Nan Meng , Hanyun Wang , Zhenyu Wang , Xinan Jiao , Jing Wang
{"title":"Occurrence and characteristics of blaOXA-181-carrying Klebsiella aerogenes from swine in China","authors":"Lin Sun ,&nbsp;Nan Meng ,&nbsp;Hanyun Wang ,&nbsp;Zhenyu Wang ,&nbsp;Xinan Jiao ,&nbsp;Jing Wang","doi":"10.1016/j.jgar.2024.04.009","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p><em>Klebsiella aerogenes</em> is a largely understudied opportunistic pathogen that can cause sepsis and lead to high mortality rates. In this study, we reported the occurrence of carbapenem-resistant <em>bla</em><sub>OXA-181</sub>-carrying <em>Klebsiella aerogenes</em> from swine in China and elucidate their genomic characteristics.</p></div><div><h3>Methods</h3><p>A total of 126 samples, including 109 swine fecal swabs, 14 environmental samples, and three feed samples were collected from a pig farm in China. The samples were enriched with LB broth culture and then inoculated into MacConkey agar plates for bacterial isolation. After PCR detection of carbapenemases genes, the <em>bla</em><sub>OXA-181</sub>-carrying isolates were subjected to antimicrobial susceptibility testing, and whole-genome sequence analysis.</p></div><div><h3>Results</h3><p>Four <em>Klebsiella aerogenes</em> isolates carrying the <em>bla</em><sub>OXA-181</sub> gene were obtained from swine faecal samples. All the 4 strains were belonged to ST438. The <em>bla</em><sub>OXA-181</sub> genes were located in IncX3-ColKP3 hybrid plasmids with the core genetic structure of IS<em>26</em>-ΔIS<em>3000</em>-ΔIS<em>Ecp1</em>-<em>bla</em><sub>OXA-181</sub>-Δ<em>lysR</em>-Δ<em>ereA</em>-Δ<em>repA</em>-IS<em>Kpn19</em>-<em>tinR</em>-<em>qnrS1</em>-ΔIS<em>2</em>-IS<em>26</em>, which suggests the potential for horizontal transfer and further dissemination of this resistance gene among <em>Enterobacteriaceae</em> and other sources.</p></div><div><h3>Conclusions</h3><p>This study represents the first instance of OXA-181-producing <em>K. aerogenes</em> being identified from swine faeces in China. It is crucial to maintain continuous monitoring and ongoing attention to the detection of <em>K. aerogenes</em> carrying <em>bla</em><sub>OXA-181</sub> and other resistance genes in pigs.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 35-41"},"PeriodicalIF":3.7000,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524000870/pdfft?md5=2b5df5485220a5c025bdb93034a6c1b8&pid=1-s2.0-S2213716524000870-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524000870","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives

Klebsiella aerogenes is a largely understudied opportunistic pathogen that can cause sepsis and lead to high mortality rates. In this study, we reported the occurrence of carbapenem-resistant blaOXA-181-carrying Klebsiella aerogenes from swine in China and elucidate their genomic characteristics.

Methods

A total of 126 samples, including 109 swine fecal swabs, 14 environmental samples, and three feed samples were collected from a pig farm in China. The samples were enriched with LB broth culture and then inoculated into MacConkey agar plates for bacterial isolation. After PCR detection of carbapenemases genes, the blaOXA-181-carrying isolates were subjected to antimicrobial susceptibility testing, and whole-genome sequence analysis.

Results

Four Klebsiella aerogenes isolates carrying the blaOXA-181 gene were obtained from swine faecal samples. All the 4 strains were belonged to ST438. The blaOXA-181 genes were located in IncX3-ColKP3 hybrid plasmids with the core genetic structure of IS26-ΔIS3000-ΔISEcp1-blaOXA-181lysRereArepA-ISKpn19-tinR-qnrS1-ΔIS2-IS26, which suggests the potential for horizontal transfer and further dissemination of this resistance gene among Enterobacteriaceae and other sources.

Conclusions

This study represents the first instance of OXA-181-producing K. aerogenes being identified from swine faeces in China. It is crucial to maintain continuous monitoring and ongoing attention to the detection of K. aerogenes carrying blaOXA-181 and other resistance genes in pigs.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
中国猪群中携带 blaOXA-181 的产气克雷伯菌的出现和特征
目的:产气克雷伯氏菌是一种未得到充分研究的机会性病原体,可引起败血症并导致高死亡率。本研究报告了中国猪群中出现的耐碳青霉烯类 blaOXA-181 产气克雷伯氏菌,并阐明了其基因组特征。样品经 LB 肉汤富集培养后接种到 MacConkey 琼脂平板进行细菌分离。结果从猪粪便样品中分离到 4 株携带 blaOXA-181 基因的产气克雷伯菌。所有 4 株菌株均属于 ST438。blaOXA-181 基因位于 IncX3-ColKP3 杂交质粒中,其核心遗传结构为 IS26-ΔIS3000-ΔISEcp1-blaOXA-181-ΔlysR-ΔereA-ΔrepA-ISKpn19-tinR-qnrS1-ΔIS2-IS26 ,这表明该耐药基因有可能在肠杆菌科和其他来源中横向转移和进一步传播。结论本研究是中国首次从猪粪便中发现产 OXA-181 的产气荚膜杆菌。对猪体内携带 blaOXA-181 和其他耐药基因的产气荚膜杆菌进行持续监测和关注至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
期刊最新文献
Surveillance and Characteristics of Vancomycin-Resistant Enterococcus Isolates in a Chinese Tertiary Hospital in Shenzhen, 2018 to 2024. Genomic analysis of IMP-8-producing Enterobacter hormaechei with a novel plasmid pK432-IMP. Monte Carlo simulation for dosage optimization of the best available therapy for bloodstream infections secondary to carbapenemase-producing Klebsiella pneumoniae in critically ill patients. Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli. From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1