Heterologous activation and metabolites identification of the pks7 gene cluster from Saccharopolyspora erythraea

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Synthetic and Systems Biotechnology Pub Date : 2024-05-09 DOI:10.1016/j.synbio.2024.05.004

Hao Tang , Xingchi Yang , Wenzong Wang , Xingjun Cui , Wenping Wei , Jing Wu , Peng Sun , Bang-Ce Ye

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Abstract

The microbial genome remains a huge treasure trove for the discovery of diverse natural products. Saccharopolyspora erythraea NRRL23338, the industry producer of erythromycin, has a dozen of biosynthetic gene clusters whose encoding products are unidentified. Heterologous expression of one of the polyketide clusters pks7 in Streptomyces albus B4 chassis resulted in the characterization of its function responsible for synthesizing both 6-methylsalicyclic acid and 6-ethylsalicyclic acid. Meanwhile, two new 6-ethylsalicyclic acid ester derivatives were isolated as shunt metabolites. Their structures were identified by comprehensive analysis of MS and NMR experiments. Putative functions of genes within the pks7 BGC were also discussed.

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红球藻 pks7 基因簇的异源激活和代谢物鉴定

微生物基因组仍然是发现各种天然产品的巨大宝库。红霉素的工业生产者 Saccharopolyspora erythraea NRRL23338 有十几个生物合成基因簇，其编码产物尚未确定。通过在白链霉菌 B4 底盘中异源表达其中一个多酮簇 pks7，确定了其合成 6-甲基水杨酸和 6-乙基水杨酸的功能。同时，还分离出两种新的 6-乙基水杨环酸酯衍生物作为分流代谢产物。通过对 MS 和 NMR 实验的综合分析，确定了它们的结构。此外，还讨论了 pks7 BGC 中基因的可能功能。

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.