Cryopreservation of bovine semen using extract of Cinnamomum zeylanicum.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-05-01 DOI:10.54680/fr24310110712

Kabir Alam, Sushant Srivastava, Bhoopendra Singh, Saurabh, Rajesh Kumar, Rabindra Kumar, Daund Sushant Sakhahari

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Abstract

BACKGROUND Antioxidants minimise oxidative stress and enhance sperm quality in the process of cryopreservation. OBJECTIVE To assess the impact of Cinnamomum zeylanicum extract as an additive during the post-dilution and post-thaw stages of Murrah buffalo semen cryopreservation. MATERIALS AND METHODS The semen sample was diluted using Tris-Egg-Yolk-Citric-Acid-Fructose-Glycerol extender and subsequently divided into three groups: Group 1, TEYCAFG without any additives or controls (C); Group 2, TEYCAFG fortified with a 50 ug/mL aqueous extract of cinnamon (T1); and Group 3, TEYCAFG fortified with a 50 ug/mL ethanolic extract of cinnamon (T2). The evaluation included an assessment of progressive motility, live spermatozoa, sperm abnormalities, HOST, CMPT, and enzyme leakage (GOT and GPT) at both the post-dilution and post-thaw stages. RESULTS The groups that received cinnamon supplementation demonstrated statistically significant improvements (p<0.05) in various parameters, including an increase in the progressive motility, live spermatozoa, and HOS-positive spermatozoa, as well as greater distance traveled by vanguard spermatozoa compared to the control group. Furthermore, the cinnamon-added groups exhibited a significant decrease (p<0.05) in the percentage of sperm abnormalities and lower enzyme leakage (GOT and GPT) in post-thawed semen. CONCLUSION Aqueous extract of C. zeylanicum at a concentration of 50 µg/mL provides superior protection of sperm structures and functions as compared to both the ethanolic extract of C. zeylanicum at the same concentration and the control group. Doi.org/10.54680/fr24310110712.

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使用肉桂提取物对牛精液进行冷冻保存。

目的评估肉桂提取物作为添加剂在稀释后和解冻后阶段对穆拉水牛精液冷冻保存的影响。材料和方法精液样本用三蛋黄-柠檬酸-果糖-甘油扩展剂稀释，然后分为三组：第 1 组，不含任何添加剂或对照组的 TEYCAFG（C）；第 2 组，添加 50 微克/毫升肉桂水提取物的 TEYCAFG（T1）；第 3 组，添加 50 微克/毫升肉桂乙醇提取物的 TEYCAFG（T2）。评估包括稀释后和解冻后阶段的精子活力、活精子、精子畸形、HOST、CMPT 和酶渗漏（GOT 和 GPT）。05），与对照组相比，包括渐进运动性、活精子和 HOS 阳性精子的增加，以及先锋精子移动距离的增加。此外，肉桂添加组的精子畸形率显著下降（p<0.05），解冻后精液中的酶渗漏（GOT 和 GPT）也更低。结论与相同浓度的肉桂乙醇提取物和对照组相比，浓度为 50 µg/mL 的肉桂水提取物能更好地保护精子的结构和功能。Doi.org/10.54680/fr24310110712.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.