Effects of Remimazolam on Cognitive Function and Nervous System in Mice

Abstract

This study investigated the safety and efficacy of remimazolam besylate by preparing remimazolam nanoemulsion. Field experiments were carried out in mice of different genders and ages. The treatment group was given intraperitoneal injection of Remimazolam nano-emulsion at different doses (0, 10, 15, 15, 20 mg/kg). The propofol group received intraperitoneal injections of propofol, while the control group received intraperitoneal injections of normal saline. The open-field of mice was detected to evaluate the effect of remimazolam on exercise response and sedation recovery time of mice. With the anesthetic effect of propofol as control, the level of P-tau phosphorylation was analyzed by westernblot, and the expression and distribution of P-tau in hippocampus was detected by immunohistochemistry. Golgi staining was used to detect the density of dendritic spines in the hippocampus. The results revealed that remimazolam could reduce the movement distance, movement speed and increase the resting time of mice. The higher the concentration of remimazolam, the stronger the sedative effect. Additionally, the inhibitory effect of low-dose rimazolam on the response of mice was the strongest in 15 min, and gradually recovered after 15 min, and the sedative effect had nothing to do with sex and sex of mice. The results of protein detection showed that compared with propofol group, remimazolam could reduce the expression and distribution of hippocampus P-tau and increase the number and density of dendritic spines. Therefore, low-dose administration of remimazolam has a short-term effectiveness, lacks toxic side effects, and provides a certain level of protection to neurological and cognitive function.