Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China.

IF 3.6 4区 医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General Virology Pub Date : 2024-05-01 DOI:10.1099/jgv.0.001989
Lin Xu, Mengyu Bie, Juan Li, Hong Zhou, Tao Hu, Michael J Carr, Liang Lu, Weifeng Shi
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Abstract

Hepeviruses have been identified in a broad range of animal hosts, including mammals, birds, and fish. In this study, rodents (n=91) from seven different species and ten pikas (Ochotona curzoniae) were collected in Qinghai Province, China. Using transcriptomic sequencing and confirmatory molecular testing, hepeviruses were detected in 27 of 45 (60 %) long-tailed dwarf hamsters (Cricetulus longicaudatus) and were undetected in other rodents and pika. The complete genome sequences from 14 representative strains were subsequently obtained, and phylogenetic analyses suggested that they represent a novel species within the genus Rocahepevirus, which we tentatively designated as Cl-2018QH. The virus was successfully isolated in human hepatoma (Huh-7) and murine fibroblast (17 Cl-1) cell lines, though both exhibited limited replication as assayed by detection of negative-sense RNA intermediates. A129 immunodeficient mice were inoculated with Cl-2018QH and the virus was consistently detected in multiple organs, despite relatively low viral loads. In summary, this study has described a novel rodent hepevirus, which enhances our knowledge of the genetic diversity of rodent hepeviruses and highlights its potential for cross-species transmission.

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中国长尾侏儒仓鼠新型啮齿类动物肝病毒的分离与鉴定
在哺乳动物、鸟类和鱼类等多种动物宿主中发现了肝病毒。本研究在中国青海省收集了来自七个不同物种的啮齿动物(n=91)和十只鼠兔(Ochotona curzoniae)。通过转录组测序和确证分子检测,在45只长尾侏儒仓鼠(Cricetulus longicaudatus)中的27只(60%)检测到了肝病毒,而在其他啮齿类动物和鼠兔中未检测到肝病毒。随后,我们获得了14株代表性毒株的完整基因组序列,系统进化分析表明,这些毒株代表了Rocahepevirus属中的一个新物种,我们暂时将其命名为Cl-2018QH。我们在人肝癌(Huh-7)和小鼠成纤维细胞(17 Cl-1)细胞系中成功分离到了该病毒,但通过检测负义 RNA 中间体,发现这两种细胞系的复制都很有限。给 A129 免疫缺陷小鼠接种 Cl-2018QH,尽管病毒载量相对较低,但仍能在多个器官中持续检测到病毒。总之,本研究描述了一种新型啮齿类动物嗜血病毒,加深了我们对啮齿类动物嗜血病毒遗传多样性的了解,并强调了其跨物种传播的潜力。
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来源期刊
Journal of General Virology
Journal of General Virology 医学-病毒学
CiteScore
7.70
自引率
2.60%
发文量
91
审稿时长
3 months
期刊介绍: JOURNAL OF GENERAL VIROLOGY (JGV), a journal of the Society for General Microbiology (SGM), publishes high-calibre research papers with high production standards, giving the journal a worldwide reputation for excellence and attracting an eminent audience.
期刊最新文献
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