Innate and adaptive immune responses in subjects with CPA secondary to post-pulmonary tuberculosis lung abnormalities.

IF 4.1 2区医学 Q1 DERMATOLOGY Mycoses Pub Date : 2024-05-01 DOI:10.1111/myc.13746

Naresh Kumar Chirumamilla, Kanika Arora, Mandeep Kaur, Ritesh Agarwal, Valliappan Muthu, Amit Rawat, Sahajal Dhooria, Kuruswamy Thurai Prasad, Ashutosh Nath Aggarwal, Shivaprakash M Rudramurthy, Arunaloke Chakrabarti, Hansraj Choudhary, Arnab Pal, Inderpaul Singh Sehgal

{"title":"Innate and adaptive immune responses in subjects with CPA secondary to post-pulmonary tuberculosis lung abnormalities.","authors":"Naresh Kumar Chirumamilla, Kanika Arora, Mandeep Kaur, Ritesh Agarwal, Valliappan Muthu, Amit Rawat, Sahajal Dhooria, Kuruswamy Thurai Prasad, Ashutosh Nath Aggarwal, Shivaprakash M Rudramurthy, Arunaloke Chakrabarti, Hansraj Choudhary, Arnab Pal, Inderpaul Singh Sehgal","doi":"10.1111/myc.13746","DOIUrl":null,"url":null,"abstract":"Background: Post-tuberculosis lung abnormality (PTLA) is the most common risk factor for chronic pulmonary aspergillosis (CPA), and 14%-25% of the subjects with PTLA develop CPA. The pathogenesis and the host immune response in subjects with PTLA who develop CPA need to be better understood.Methods: We prospectively compared the innate and adaptive immune responses mounted by patients of PTLA with or without CPA (controls). We studied the neutrophil oxidative burst (by dihydrorhodamine 123 test), classic (serum C3 and C4 levels) and alternative (mannose-binding lectin [MBL] protein levels) complement pathway, serum immunoglobulins (IgG, IgM and IgA), B and T lymphocytes and their subsets in subjects with PTLA with or without CPA.Results: We included 111 subjects (58 CPA and 53 controls) in the current study. The mean ± SD age of the study population was 42.6 ± 15.7 years. The cases and controls were matched for age, gender distribution and body weight. Subjects with CPA had impaired neutrophil oxidative burst, lower memory T lymphocytes and impaired Th-1 immune response (lower Th-1 lymphocytes) than controls. We found no significant difference between the two groups in the serum complement levels, MBL levels, B-cell subsets and other T lymphocyte subsets.Conclusion: Subjects with CPA secondary to PTLA have impaired neutrophil oxidative burst and a lower Th-1 response than controls.","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"67 5","pages":"e13746"},"PeriodicalIF":4.1000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoses","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/myc.13746","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Post-tuberculosis lung abnormality (PTLA) is the most common risk factor for chronic pulmonary aspergillosis (CPA), and 14%-25% of the subjects with PTLA develop CPA. The pathogenesis and the host immune response in subjects with PTLA who develop CPA need to be better understood.

Methods: We prospectively compared the innate and adaptive immune responses mounted by patients of PTLA with or without CPA (controls). We studied the neutrophil oxidative burst (by dihydrorhodamine 123 test), classic (serum C3 and C4 levels) and alternative (mannose-binding lectin [MBL] protein levels) complement pathway, serum immunoglobulins (IgG, IgM and IgA), B and T lymphocytes and their subsets in subjects with PTLA with or without CPA.

Results: We included 111 subjects (58 CPA and 53 controls) in the current study. The mean ± SD age of the study population was 42.6 ± 15.7 years. The cases and controls were matched for age, gender distribution and body weight. Subjects with CPA had impaired neutrophil oxidative burst, lower memory T lymphocytes and impaired Th-1 immune response (lower Th-1 lymphocytes) than controls. We found no significant difference between the two groups in the serum complement levels, MBL levels, B-cell subsets and other T lymphocyte subsets.

Conclusion: Subjects with CPA secondary to PTLA have impaired neutrophil oxidative burst and a lower Th-1 response than controls.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

继发于肺结核后肺部异常的 CPA 患者的先天性和适应性免疫反应。

背景：结核病后肺部异常（PTLA）是慢性肺曲霉菌病（CPA）最常见的危险因素，14%-25%的PTLA患者会发展为CPA。我们需要更好地了解 PTLA 患者发展为 CPA 的发病机制和宿主免疫反应：我们对患有或未患有 CPA 的 PTLA 患者（对照组）的先天性免疫反应和适应性免疫反应进行了前瞻性比较。我们研究了伴有或不伴有 CPA 的 PTLA 患者的中性粒细胞氧化爆发（通过二氢罗丹明 123 试验）、经典补体途径（血清 C3 和 C4 水平）和替代补体途径（甘露糖结合凝集素 [MBL] 蛋白水平）、血清免疫球蛋白（IgG、IgM 和 IgA）、B 淋巴细胞和 T 淋巴细胞及其亚群：本研究共纳入 111 名受试者（58 名 CPA 和 53 名对照组）。研究对象的平均年龄为（42.6±15.7）岁。病例和对照组的年龄、性别分布和体重均匹配。与对照组相比，CPA 患者的中性粒细胞氧化爆发受损，记忆 T 淋巴细胞减少，Th-1 免疫反应受损（Th-1 淋巴细胞减少）。我们发现两组患者在血清补体水平、MBL水平、B细胞亚群和其他T淋巴细胞亚群方面没有明显差异：结论：与对照组相比，继发于 PTLA 的 CPA 患者的中性粒细胞氧化爆发受损，Th-1 反应较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Mycoses 医学-皮肤病学

CiteScore

10.00

自引率

8.20%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi. Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.