In-vivo evaluation of the adverse effects of ochratoxin A on broiler chicken health and adsorption efficacy of indigenous and commercial clay of Balochistan, Pakistan.
Mustafa Rahim Abro, Nadeem Rashid, Khanoranga, Zainab Siddique
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引用次数: 0
Abstract
Mycotoxins in animal feed pose health risks and economic losses, but using various adsorbent types could potentially protect animals from mycotoxicosis. The study aimed to assess the effect of OTA on the health of broiler chickens and to envisage the ameliorative potential of clay adsorbents. The objectives of this in vivo study were to investigate the effects of OTA on productivity, biochemical parameters, fecal residues, and the preventive effects of indigenous and commercial clay of Balochistan as adsorbents to alleviate the adverse effects of exposure. Male broiler chickens (n = 160) were treated with 400 μg/kg OTA and 0.5 g/kg clay adsorbent for 42 days, with feed and water available in an ad libitum manner. The amount of OTA in diet and fecal residues was assessed through HPLC. The administration of OTA in the diet, resulted in a significant (p < 0.05) decrease in the average daily gain (ADG) and average daily feed intake (ADFI) while increasing the feed conversion ratio (FCR) as compared to the control group. Furthermore, no significant (p > 0.05) differences were found between the weight gain of broiler chickens fed without OTA (positive control) and that of chickens fed adsorbent. The group given a diet containing OTA without adsorbents as compared to the control and adsorbent-supplemented group has shown a significant (p < 0.05) increase in the relative weight of the liver, kidney, gizzard, and proventriculus while decreasing the relative weight of the spleen and bursa of Fabricius. Alterations in the levels of serum total protein (TP), cholesterol (CHL), serum urea (SU), enzymatic activity (aspartate aminotransferase (AST) and alanine transaminase (ALT)), and creatinine were observed in the OTA-intoxicated and adsorbent-supplemented groups as compared to the control group. Adsorbent supplementation resulted in a significantly (p < 0.05) higher OTA content in the faeces. It can be concluded from the results of this study, that OTA intoxication negatively affects the health of broiler chickens, and the clay of Balochistan has shown effective adsorption potential against OTA.
动物饲料中的霉菌毒素会带来健康风险和经济损失，但使用各种类型的吸附剂有可能保护动物免受霉菌毒素中毒的危害。这项研究旨在评估 OTA 对肉鸡健康的影响，并设想粘土吸附剂的改善潜力。这项体内研究的目的是调查 OTA 对生产率、生化参数、粪便残留物的影响，以及俾路支省本地粘土和商用粘土作为吸附剂对减轻接触 OTA 的不良影响的预防作用。雄性肉鸡（n = 160）连续 42 天接受 400 μg/kg OTA 和 0.5 g/kg 粘土吸附剂的处理，饲料和水自由供给。通过高效液相色谱法评估日粮和粪便中的 OTA 残留量。日粮中添加 OTA 后，未添加 OTA 的肉鸡（阳性对照组）与添加吸附剂的肉鸡的增重差异显著（p p > 0.05）。与对照组和添加吸附剂组相比，饲喂含有 OTA 且不含吸附剂的日粮组的增重显著（P
期刊介绍：
Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.
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