The Increased Prevalence of rmpA Gene in Klebsiella pneumoniae Isolates Coharboring bla_NDM and bla_OXA-48-like Genes.

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES Microbial drug resistance Pub Date : 2024-08-01 Epub Date: 2024-05-21 DOI:10.1089/mdr.2023.0296

Nora M El Naggar, Riham M Shawky, Fathy M E Serry, Mohamed Emara

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Abstract

The emergence of carbapenemase-producing Klebsiella pneumoniae poses a substantial risk to public health. It is essential to comprehend the influence of carbapenemase on the virulence characteristics of K. pneumoniae in order to devise successful strategies for combating these infections. In this study, we explored the distribution disparity of virulence determinants between carbapenemase-producing (CP-Kp, n = 52) and carbapenemase-nonproducing (CN-Kp, n = 43) isolates. The presence of carbapenemases was detected via the modified carbapenem inactivation method and confirmed by PCR. The New Delhi metallo-β-lactamase (bla_NDM) and Oxacillinase-48-like (bla_OXA-48-like) genes were the most prevalent (94.23% and 76.92%, respectively) in CP-Kp isolates. Coexistence of bla_NDM and bla_OXA-48-like was observed in 71.15% of isolates, whereas 5.77% coharbored bla_NDM and bla_KPC. PCR analysis revealed the presence of several virulence genes, including adhesins (fimH, 92.63%, mrkD, 97.89%), capsule-associated virulence (uge, 90.53%), the K2 capsule serotype (k2, 6.32%), the iron acquisition system (kfu, 23.16%), and the regulator of mucoid phenotype (rmpA, 28.42%). A significantly higher prevalence of rmpA was detected in the CP-Kp compared with the CN-Kp (24/52 vs. 3/43, p < 0.0001), indicating a potential association between rmpA and carbapenemase acquisition. In addition, the majority of rmpA (22/24) positive isolates in the CP-Kp isolates coharbored bla_NDM and either bla_OXA-48-like or bla_KPC.

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肺炎克雷伯菌分离株中同时携带 blaNDM 和 blaOXA-48 类基因的 rmpA 基因的流行率增加。

产碳青霉烯酶肺炎克雷伯氏菌的出现对公共卫生构成了巨大风险。了解碳青霉烯酶对肺炎克雷伯氏菌毒力特征的影响对于制定成功的抗感染策略至关重要。在本研究中，我们探讨了产碳青霉烯酶（CP-Kp，n = 52）和不产碳青霉烯酶（CN-Kp，n = 43）分离株之间毒力决定因素的分布差异。碳青霉烯酶的存在是通过改良的碳青霉烯灭活法检测到的，并通过 PCR 进行了确认。在 CP-Kp 分离物中，新德里金属-β-内酰胺酶（blaNDM）和类奥沙西林酶-48（blaOXA-48-like）基因最为普遍（分别占 94.23% 和 76.92%）。71.15%的分离物中观察到 blaNDM 和 blaOXA-48-like 基因共存，而 5.77% 的分离物中 blaNDM 和 blaKPC 基因共存。PCR 分析显示，分离物中存在多种毒力基因，包括粘附素（fimH，92.63%；mrkD，97.89%）、胶囊相关毒力（uge，90.53%）、K2 胶囊血清型（k2，6.32%）、铁获取系统（kfu，23.16%）和粘液表型调节器（rmpA，28.42%）。与 CN-Kp 相比，CP-Kp 中检测到的 rmpA 患病率明显更高（24/52 对 3/43，p < 0.0001），这表明 rmpA 与碳青霉烯酶的获得之间可能存在关联。此外，在 CP-Kp 分离物中，大多数 rmpA 阳性分离物（22/24）都带有 blaNDM 和 blaOXA-48-like 或 blaKPC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.