Sequence reliance of the Drosophila context-dependent transcription factor CLAMP.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY Genetics Pub Date : 2024-07-08 DOI:10.1093/genetics/iyae060

Lauren J Hodkinson, Julia Gross, Casey A Schmidt, Pamela P Diaz-Saldana, Tsutomo Aoki, Leila E Rieder

引用次数: 0

Abstract

Despite binding similar cis elements in multiple locations, a single transcription factor (TF) often performs context-dependent functions at different loci. How factors integrate cis sequence and genomic context is still poorly understood and has implications for off-target effects in genetic engineering. The Drosophila context-dependent TF chromatin-linked adaptor for male-specific lethal proteins (CLAMP) targets similar GA-rich cis elements on the X-chromosome and at the histone gene locus but recruits very different, locus-specific factors. We discover that CLAMP leverages information from both cis element and local sequence to perform context-specific functions. Our observations imply the importance of other cues, including protein-protein interactions and the presence of additional cofactors.

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果蝇上下文依赖性转录因子 CLAMP 的序列依赖性。

尽管单个转录因子（TF）在多个位置与相似的顺式元件结合，但它往往会在不同的位点上执行依赖于上下文的功能。人们对转录因子如何整合顺式序列和基因组上下文还知之甚少，这对基因工程中的脱靶效应也有影响。果蝇的上下文依赖性 TF 染色质连接适配器雄性特异性致死蛋白（CLAMP）以 X 染色体和组蛋白基因座上类似的 GA 丰富顺式元件为目标，但却招募了非常不同的基因座特异性因子。我们发现，CLAMP利用顺式元件和局部序列的信息来执行特定的功能。我们的观察结果表明了其他线索的重要性，包括蛋白质与蛋白质之间的相互作用以及其他辅助因子的存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.