Timing is everything: advances in quantifying splicing kinetics.

IF 13 1区 生物学 Q1 CELL BIOLOGY Trends in Cell Biology Pub Date : 2024-11-01 Epub Date: 2024-05-21 DOI:10.1016/j.tcb.2024.03.007
Hope E Merens, Karine Choquet, Autum R Baxter-Koenigs, L Stirling Churchman
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Abstract

Splicing is a highly regulated process critical for proper pre-mRNA maturation and the maintenance of a healthy cellular environment. Splicing events are impacted by ongoing transcription, neighboring splicing events, and cis and trans regulatory factors on the respective pre-mRNA transcript. Within this complex regulatory environment, splicing kinetics have the potential to influence splicing outcomes but have historically been challenging to study in vivo. In this review, we highlight recent technological advancements that have enabled measurements of global splicing kinetics and of the variability of splicing kinetics at single introns. We demonstrate how identifying features that are correlated with splicing kinetics has increased our ability to form potential models for how splicing kinetics may be regulated in vivo.

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时间就是一切:量化剪接动力学的进展。
剪接是一个受到高度调控的过程,对于前核糖核酸的正常成熟和维持健康的细胞环境至关重要。剪接事件受到正在进行的转录、相邻剪接事件以及相应前 mRNA 转录本上顺式和反式调控因子的影响。在这种复杂的调控环境中,剪接动力学有可能影响剪接结果,但体内研究历来具有挑战性。在这篇综述中,我们将重点介绍最近的技术进步,这些技术进步使我们能够测量全局剪接动力学和单个内含子剪接动力学的变异性。我们展示了如何通过识别与剪接动力学相关的特征来提高我们建立潜在模型的能力,以了解剪接动力学在体内是如何被调控的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Trends in Cell Biology
Trends in Cell Biology 生物-细胞生物学
CiteScore
32.00
自引率
0.50%
发文量
160
审稿时长
61 days
期刊介绍: Trends in Cell Biology stands as a prominent review journal in molecular and cell biology. Monthly review articles track the current breadth and depth of research in cell biology, reporting on emerging developments and integrating various methods, disciplines, and principles. Beyond Reviews, the journal features Opinion articles that follow trends, offer innovative ideas, and provide insights into the implications of new developments, suggesting future directions. All articles are commissioned from leading scientists and undergo rigorous peer-review to ensure balance and accuracy.
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