Pedro Nacib Jorge-Neto , Fabio de Moraes Francisco , Mario Davi Dias Carneiro , Sergio Ricardo Brito Santos , Letícia Alecho Requena , Sofia Dressel Ramos , Matheus Felix de Goés , Rafael Franco Valle , Fabiana Lucia André Padilha , Helen Colbachini , Rafael Caprioli Gutierrez , Larissa Schneider Brandão Souza , Verônica Takatsuka Manoel , Laura Chrispim Reisfeld , Thyara Deco-Souza , Roberta Ferreira Leite , Cristiane Schilbach Pizzutto
{"title":"Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm","authors":"Pedro Nacib Jorge-Neto , Fabio de Moraes Francisco , Mario Davi Dias Carneiro , Sergio Ricardo Brito Santos , Letícia Alecho Requena , Sofia Dressel Ramos , Matheus Felix de Goés , Rafael Franco Valle , Fabiana Lucia André Padilha , Helen Colbachini , Rafael Caprioli Gutierrez , Larissa Schneider Brandão Souza , Verônica Takatsuka Manoel , Laura Chrispim Reisfeld , Thyara Deco-Souza , Roberta Ferreira Leite , Cristiane Schilbach Pizzutto","doi":"10.1016/j.therwi.2024.100091","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>One-third of elasmobranch species are threatened with extinction, and the precise assessment of their sperm parameters and quality is one stage in the development of reproductive technology for animal conservation.</p></span></li></ul><ul><li><span>2.</span><span><p>Computer-Assisted Sperm Analysis (CASA) is the gold standard for sperm analysis; however, there were not one setup established for elasmobranchs.</p></span></li></ul><p></p><ul><li><span>3.</span><span><p>This study presents the description of a specific setup developed for elasmobranch semen analysis using the CASA system.</p></span></li></ul><ul><li><span>4.</span><span><p>In addition, it provides a gold-standard technique protocol for elasmobranch sperm quality assessment.</p></span></li></ul><p></p><ul><li><span>5.</span><span><p>The described setup presented an accuracy of more than 90 percent in fourteen distinct species of freshwater and marine sharks, stingrays, and rays, failing only when there were two or more bundled sperm.</p></span></li></ul></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000229/pdfft?md5=e82e59bee8065b6a7446d1f238acb25b&pid=1-s2.0-S2773093X24000229-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theriogenology wild","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2773093X24000229","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
1.
One-third of elasmobranch species are threatened with extinction, and the precise assessment of their sperm parameters and quality is one stage in the development of reproductive technology for animal conservation.
2.
Computer-Assisted Sperm Analysis (CASA) is the gold standard for sperm analysis; however, there were not one setup established for elasmobranchs.
3.
This study presents the description of a specific setup developed for elasmobranch semen analysis using the CASA system.
4.
In addition, it provides a gold-standard technique protocol for elasmobranch sperm quality assessment.
5.
The described setup presented an accuracy of more than 90 percent in fourteen distinct species of freshwater and marine sharks, stingrays, and rays, failing only when there were two or more bundled sperm.
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