Evaluation of phenylurea-based antimicrobial agent in natural rubber latex dipped film as an application for medical gloves

Abstract

This paper describes the effects of incorporating the commercially available phenylurea-based antimicrobial agent S89 to pre-vulcanised natural rubber latex (NRL). The physical properties of the dipped film derived from the S89 incorporated into pre-vulcanised NRL such as antimicrobial efficacy, wettability properties, surface characteristics, surface morphology and tensile strength were further evaluated. The antibacterial activity of S89 in NRL films was tested using the agar disc diffusion method and S89 had been demonstrated to suppress and prevent bacterial growth even at low loading levels. Moreover, the minimum inhibitory concentration (MIC) of S89 was obtained by fitting the inhibition zone diameter and loading of S89 using two mathematical diffusion models, free and dissipative models. In this work, the dissipative model is shown to be the best-described diffusion model for S89. The leaching behaviour of S89 in an artificial sweat was investigated where the leachate of S89 is observed after 20 min of immersion at a very low concentration (0.039 ug/mL). Interestingly, NRL films incorporated with S89 displayed better wettability properties on air facing (AF) surface indicating that the AF surface of the film had been altered to be more hydrophilic. Nonetheless, there were no changes observed to the former facing (FF) surface. In addition, the polarity of the surface was also reduced which corresponds to the contact angle results. Further analysis of the Field Emission Scanning Electron Microscopy (FESEM) surface morphology on the NRL film incorporated with S89 exhibited sufficient miscibility and homogeneity during latex compounding with no agglomeration observed on both surfaces (air and former) and at the cross-sectional surface. Meanwhile, comparative tensile strength (of more than 20Mpa) of NRL films incorporated with S89 was observed for low dosage at 1 phr and 3 phr. In conclusion, antimicrobial natural rubber (NR) films for glove application with satisfactory tensile properties, adequate wettability properties, non-agglomerated surface morphology and good antimicrobial properties were achieved by employing a low loading of S89 in the NRL.