A Novel Detection Method of Fe(II) Based on Nanopore Sensor

Abstract

A novel Fe(II) detection method was developed on nanopore platform in this work. The incubation mixture of dumbbell DNA and Bleomycin (BLM) wounld produce blockage signals on nanopore sensor. The addition of Fe(II) would generate BLM·Fe(II) to destroy the dumbbell DNA molecules and release short-strand oligonucleotide fragments which can produce spiked current signals on nanopore sensor. The statistical analysis shows the intervals between adjacent blockage signals decrease with elevated concentration of Fe(II), and a linear relationship between the event intervals versus the Fe(II) concentration is confirmed in the range of 0.1 to 500 nM with detection limit as low as 0.12 nM. Varied kinds of metal ions were examined for comparison, demonstrating high selectivity for Fe(II) detection. The proposed method may be potentially applied for qualitative and quantitative detection of Fe(II) due to its high sensitivity and selectivity.