Proteolysis of mitochondrial-coded and nuclear-coded proteins found in yeast mitochondria.

G Jacob, R Téllez, W Torres, R Ocasio, C Basilio, C George-Nascimento
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Abstract

The rate of degradation of radioactive labeled mitochondrial proteins synthesized both in vitro and in vivo by isolated yeast mitochondria and growing yeast cells respectively, has been studied. It was found that the in vitro-synthesized mitochondrial proteins are rapidly degraded by an energy-dependent proteolytic system. Under the same experimental conditions the in vivo-synthesized mitochondrial proteins are slowly degraded to a limited extent by a protease which is slightly inhibited by ATP. During this period, the mitochondria are coupled and metabolically active. It is proposed that mitochondria possess an energy-dependent proteolytic system that recognizes as substrates either "abnormal" proteins or unassembled protein subunits encoded in the mitochondrial genome. An apparently different system, which is independent of energy, seems to be responsible for the slow and limited degradation of "normal" mitochondrial proteins.

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酵母线粒体中发现的线粒体编码蛋白和核编码蛋白的蛋白质水解。
研究了分离酵母线粒体和生长酵母细胞在体外和体内合成的放射性标记线粒体蛋白的降解速率。研究发现,体外合成的线粒体蛋白被能量依赖性蛋白水解系统迅速降解。在相同的实验条件下,体内合成的线粒体蛋白被蛋白酶缓慢地降解到有限的程度,蛋白酶被ATP轻微抑制。在此期间,线粒体偶联并且代谢活跃。有人提出,线粒体具有一个能量依赖的蛋白质水解系统,该系统可以识别线粒体基因组中编码的“异常”蛋白质或未组装的蛋白质亚基作为底物。一个明显不同的系统,它独立于能量,似乎是“正常”线粒体蛋白质缓慢而有限的降解的原因。
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The evolution of hexokinases. [Hermann Niemeyer Fernández (1918-1991) and science in Chile]. Society of Biology of Chile and the Associated Societies, 34th annual meeting. Puyehue, Chile, 27-30 November 1991. Abstracts. [Preparation and characterization of a monoclonal peroxidase-antiperoxidase complex]. [Neurochemical substrate of the behavioral pharmacology of ethanol].
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