Lan Hai Anh Tran, Lauren A. Lowe, Yaam Deckel, Matthew Turner, James Luong, Omar Abdullah A Khamis, Megan L. Amos, Anna Wang
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引用次数: 0
Abstract
We report efforts to quantify the loading of cell-sized lipid vesicles using in-line digital holographic microscopy. This method does not require fluorescent reporters, fluorescent tracers, or radioactive tracers. A single-color LED light source takes the place of conventional illumination to generate holograms rather than bright field images. By modeling the vesicle’s scattering in a microscope with a Lorenz–Mie light scattering model and comparing the results to data holograms, we are able to measure the vesicle’s refractive index and thus loading. Performing the same comparison for bulk light scattering measurements enables the retrieval of vesicle loading for nanoscale vesicles.
我们报告了利用在线数字全息显微镜量化细胞大小脂质囊泡负载的工作。这种方法不需要荧光报告物、荧光示踪剂或放射性示踪剂。单色 LED 光源取代传统照明，生成全息图像而不是明视野图像。通过在显微镜中使用洛伦兹-米光散射模型对囊泡散射进行建模，并将结果与数据全息图进行比较，我们就能测量囊泡的折射率，从而测量载荷。对体光散射测量进行同样的比较，就能检索出纳米级囊泡的载荷。
期刊介绍：
ACS Physical Chemistry Au is an open access journal which publishes original fundamental and applied research on all aspects of physical chemistry. The journal publishes new and original experimental computational and theoretical research of interest to physical chemists biophysical chemists chemical physicists physicists material scientists and engineers. An essential criterion for acceptance is that the manuscript provides new physical insight or develops new tools and methods of general interest. Some major topical areas include:Molecules Clusters and Aerosols; Biophysics Biomaterials Liquids and Soft Matter; Energy Materials and Catalysis
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