Palmdelphin inhibits ovarian cancer cell stem specification via downregulating ring finger protein 145

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-05-01 DOI:10.1615/critreveukaryotgeneexpr.2024053542

Nanzi Xie, Sisi Yang, Changlan Dai, Wei Chen

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Abstract

This study aimed to investigate the roles of PALMD in ovarian cancer. mRNA expression was detected using RT-qPCR. The aldehyde dehydrogenase (ALDH) activity and biomarkers of ovarian cancer stem cells were determined using flow cytometry. The stemness of ovarian cancer cells was determined using sphere formation assay. Cell viability was determined using CCK-8 assay. The number of colonies was determined using colony formation assay. Cell migration was detected using wound healing assay. Cell invasion was determined using transwell assay. The results showed that PALMD was downregulated in ovarian cancer. Overexpressed PALMD inhibited the proliferative, migrative and invasive ability of ovarian cancer cells. Moreover, PALMD inhibited the stem-like properties of ovarian cancer cells. Additionally, PALMD downregulated ring finger protein 145 (RNF145) expression, overexpression of which contributed to the aggressiveness of ovarian cancer cells. Taken together, PALMD suppressed ovarian cancer cell stem specification via inhibiting RNF145 expression.

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掌叶素通过下调环指蛋白145抑制卵巢癌细胞干的分化

本研究旨在探讨 PALMD 在卵巢癌中的作用。使用流式细胞术测定了卵巢癌干细胞的醛脱氢酶（ALDH）活性和生物标志物。卵巢癌细胞的干性是通过球形成试验确定的。使用 CCK-8 检测法确定细胞活力。使用集落形成试验测定集落数量。使用伤口愈合试验检测细胞迁移。细胞侵袭采用透孔试验检测。结果表明，PALMD在卵巢癌中被下调。过表达的 PALMD 可抑制卵巢癌细胞的增殖、迁移和侵袭能力。此外，PALMD 还能抑制卵巢癌细胞的干样特性。此外，PALMD 还能下调环指蛋白 145（RNF145）的表达，而环指蛋白 145 的过度表达会增加卵巢癌细胞的侵袭性。综上所述，PALMD 通过抑制 RNF145 的表达来抑制卵巢癌细胞的干细胞分化。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

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审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.