D-Ribose-L-Cysteine and L-Dopa Co-Supplementation Attenuates Paraquat-Induced Oxidative Damage and Locomotion Deficit in Drosophila melanogaster

IF 0.5 4区医学 Q4 NEUROSCIENCES Neurochemical Journal Pub Date : 2024-05-24 DOI:10.1134/s1819712424010021

Adeola Oluwatosin Adedara, Grace Temitope Akingbade

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Abstract

D-ribose L-cysteine is a glutathione precursor that acts as an antioxidant. In the present study, we investigated the effect of D-ribose L-cysteine and L-dopa combination against paraquat toxicity in Drosophila melanogaster. Adult wild-type flies were exposed to Paraquat (500 μM), RLC (250 μM), and/or L-dopa (0.1 mM) in their diet for 7 days. The survival rate, locomotion activities, and markers of antioxidants (Catalase, glutathione-S-transferase, superoxide dismutase, thioredoxin reductase, total and non-protein thiol), oxidative stress (hydrogen peroxide, protein carbonyl, malondialdehyde, nitrite/nitrates), and dehydrogenase activity were measured in the flies. The results showed a non-toxic effect of the combination of RLC and L-dopa. In addition, elevated oxidative stress markers due to paraquat treatments were significantly reduced after treatments with RLC and L-dopa. Combination treatment also significantly restored altered antioxidant status, improved survival rate, and impaired locomotion induced by paraquat. This study suggests that RLC could serve as adjunct therapy with L-dopa in managing Parkinson’s disease and toxicity associated with paraquat and related compounds

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同时补充 D-核糖-L-半胱氨酸和左旋多巴可减轻百草枯诱导的黑腹果蝇氧化损伤和运动障碍

摘要 D-核糖-L-半胱氨酸是一种谷胱甘肽前体，具有抗氧化作用。本研究探讨了D-核糖-L-半胱氨酸和左旋多巴联合使用对黑腹果蝇百草枯毒性的影响。将成年野生型果蝇置于百草枯（500 μM）、RLC（250 μM）和/或 L-多巴（0.1 mM）的食物中 7 天。对苍蝇的存活率、运动活性、抗氧化剂指标（过氧化氢酶、谷胱甘肽-S-转移酶、超氧化物歧化酶、硫氧还原酶、总硫醇和非蛋白质硫醇）、氧化应激指标（过氧化氢、蛋白质羰基、丙二醛、亚硝酸盐/硝酸盐）和脱氢酶活性进行了测定。结果显示，RLC 和左旋多巴的组合无毒性作用。此外，在使用 RLC 和左旋多巴处理后，百草枯引起的氧化应激指标升高明显降低。联合治疗还能明显恢复百草枯引起的抗氧化状态改变、提高存活率和运动障碍。这项研究表明，RLC 可作为左旋多巴的辅助疗法，用于治疗帕金森病以及与百草枯和相关化合物有关的毒性。

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来源期刊

Neurochemical Journal 医学-神经科学

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components. The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.