{"title":"The Ameliorative Effects of Naringin on Cisplatin-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells","authors":"Pinar Bayram, Selina Aksak Karamese","doi":"10.1134/s1819712424010094","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this study, our aim was to investigate the potential therapeutic effects of different doses of naringin on SH-SY5Y neuroblastoma cells by effecting the 3 different cellular processes: (i) apoptosis, (ii) inflammation, and (iii) oxidative stress. For this reason, we performed ELISA to detect the levels/positivity of IL-1-beta, IL-6, IL-10, TGF-beta, SOD, CAT, LPO, VEGF, caspase-3 and caspase-9. Our study was designed as groups in which control, only cisplatin, only naringin and cisplatin with naringin co-incubated groups. In cisplatin and naringin co-incubated groups, cisplatin was applied on SH-SY5Y neuroblastoma cells for 2 h and then co-incubated with appropriate doses of naringin. In the without cisplatin groups, only naringin was administered. CVDK-8 was used for cell viability and FDA-PI immunofluorescent dyes were used to determine the ratio of dead and viable cells. For ELISA; the culture media of all experimental groups were collected and the levels of TGF-beta, IL-10, IL-6 and IL-1-beta, caspase-3, caspase-9, VEGF, SOD, CAT and LPO were determined. Immunofluorescence staining results were also in line with the viability analysis results and neurotoxicity was inhibited at the best 25 µM dose. When the ELISA results are examined, it was seen that Cisplatin caused an important increase in the levels of pro-inflammatory cytokines, caspase-3, caspase-9, and the activities of SOD, CAT, LPO enzymes, while naringin application reduced the levels of mentioned parameters in different doses. Naringin was able to balance the activities of oxidative and anti-oxidant enzymes, suppress inflammation, apoptosis and metastasis. Although it has various beneficial effects on neurotoxicity models, further molecular studies should be performed for better understanding the mechanism of naringin effects.</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s1819712424010094","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, our aim was to investigate the potential therapeutic effects of different doses of naringin on SH-SY5Y neuroblastoma cells by effecting the 3 different cellular processes: (i) apoptosis, (ii) inflammation, and (iii) oxidative stress. For this reason, we performed ELISA to detect the levels/positivity of IL-1-beta, IL-6, IL-10, TGF-beta, SOD, CAT, LPO, VEGF, caspase-3 and caspase-9. Our study was designed as groups in which control, only cisplatin, only naringin and cisplatin with naringin co-incubated groups. In cisplatin and naringin co-incubated groups, cisplatin was applied on SH-SY5Y neuroblastoma cells for 2 h and then co-incubated with appropriate doses of naringin. In the without cisplatin groups, only naringin was administered. CVDK-8 was used for cell viability and FDA-PI immunofluorescent dyes were used to determine the ratio of dead and viable cells. For ELISA; the culture media of all experimental groups were collected and the levels of TGF-beta, IL-10, IL-6 and IL-1-beta, caspase-3, caspase-9, VEGF, SOD, CAT and LPO were determined. Immunofluorescence staining results were also in line with the viability analysis results and neurotoxicity was inhibited at the best 25 µM dose. When the ELISA results are examined, it was seen that Cisplatin caused an important increase in the levels of pro-inflammatory cytokines, caspase-3, caspase-9, and the activities of SOD, CAT, LPO enzymes, while naringin application reduced the levels of mentioned parameters in different doses. Naringin was able to balance the activities of oxidative and anti-oxidant enzymes, suppress inflammation, apoptosis and metastasis. Although it has various beneficial effects on neurotoxicity models, further molecular studies should be performed for better understanding the mechanism of naringin effects.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
