{"title":"Reduction of BRCA1 Induced by Sevoflurane Exacerbates the Neurotoxicity of Amyloid-β","authors":"Zhenghua Wu, Kuan Huang","doi":"10.1134/s1819712424010264","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Inhaled anesthetic sevoflurane is widely reported to induce neurotoxicity, whereas the underlying mechanism is less elucidated. Here, we investigated that sevoflurane anesthesia causes a decrease in learning and memory functions, especially in aged mice. BRCA1 was found to be downregulated by sevoflurane administration in the hippocampus. Sevoflurane exposure led to decreased BRCA1 in vitro, accompanied by an increase of β-amyloid precursor protein and β-amyloid. Aβ1-42 suppressed the protein abundance of BRCA1 but not mRNA, and BRCA1 could not alter the expression of β-amyloid. Furthermore, BRCA1 was observed to participate in the neurotoxicity induced by sevoflurane, since BRCA1 knockdown exacerbated, while overexpression mitigated sevoflurane-induced decrease of cell viability of neuronal cells. Our results demonstrated for the first time that the vital role of BRCA1 in sevoflurane-induced neurotoxicity, and β‑amyloid might serve as the link between BRCA1 and sevoflurane.</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s1819712424010264","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Inhaled anesthetic sevoflurane is widely reported to induce neurotoxicity, whereas the underlying mechanism is less elucidated. Here, we investigated that sevoflurane anesthesia causes a decrease in learning and memory functions, especially in aged mice. BRCA1 was found to be downregulated by sevoflurane administration in the hippocampus. Sevoflurane exposure led to decreased BRCA1 in vitro, accompanied by an increase of β-amyloid precursor protein and β-amyloid. Aβ1-42 suppressed the protein abundance of BRCA1 but not mRNA, and BRCA1 could not alter the expression of β-amyloid. Furthermore, BRCA1 was observed to participate in the neurotoxicity induced by sevoflurane, since BRCA1 knockdown exacerbated, while overexpression mitigated sevoflurane-induced decrease of cell viability of neuronal cells. Our results demonstrated for the first time that the vital role of BRCA1 in sevoflurane-induced neurotoxicity, and β‑amyloid might serve as the link between BRCA1 and sevoflurane.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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