Evolutionary dynamics of topotype ME-SA/Ind-2001 of foot and mouth disease virus serotype-O in Pakistan: 2017-2022.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Iranian journal of veterinary research Pub Date : 2023-01-01 DOI:10.22099/IJVR.2023.47837.6940

A Munir, A A Anjum, I Altaf, A R Awan

{"title":"Evolutionary dynamics of topotype ME-SA/Ind-2001 of foot and mouth disease virus serotype-O in Pakistan: 2017-2022.","authors":"A Munir, A A Anjum, I Altaf, A R Awan","doi":"10.22099/IJVR.2023.47837.6940","DOIUrl":null,"url":null,"abstract":"Background: During past few years, the Ind-2001 lineage of the Middle East-South Asia topotype (ME-SA) of the foot-and-mouth disease (FMD) virus has been implicated in FMD outbreaks in Pakistan.Aims: This work conducts a comprehensive evolutionary analysis of the Ind-2001 and Pan Asia II lineages, with a specific emphasis on their geographical distribution, lineage classification, and sub-lineage distribution within the region. Furthermore, it aims to expand our understanding of the conserved region of the VP1 protein.Methods: Total samples (n=50) were subjected to antigen detection ELISA and RT-PCR for serotype determination. Confirmed serotype-O isolates (n=17) underwent sequencing for lineage comparison, mutation impact assessment on the VP1 protein GH loop, 3D structure prediction, and further comparative analysis.Results: Isolates collected from 2017 to 2020 were identified as serotypes O/ME-SA/Pan Asia II ANT10 and O/ME-SA/Pak14. Notably, isolates collected from 2020 to 2022 belonged to a novel FMDV serotype O/ME-SA/Ind-2001e lineage. Phylogenetic analyses indicated that these strains were distinct from dominant contemporaneous strains which may challenge Pakistan's FMD control measures. These isolates exhibited variance in the VP1 epitope, specifically in amino acid residues 135-155, known to influence neutralizing antibody generation.Conclusion: Observed mutations suggest potential challenges to current vaccination efficacy against FMD. This emphasizes enhanced FMD surveillance and demonstrates that tracking the emergence of the O/ME-SA/Ind-2001e lineage is important for determining FMD control strategies in Asia.","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"24 4","pages":"339-344"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127728/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2023.47837.6940","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: During past few years, the Ind-2001 lineage of the Middle East-South Asia topotype (ME-SA) of the foot-and-mouth disease (FMD) virus has been implicated in FMD outbreaks in Pakistan.

Aims: This work conducts a comprehensive evolutionary analysis of the Ind-2001 and Pan Asia II lineages, with a specific emphasis on their geographical distribution, lineage classification, and sub-lineage distribution within the region. Furthermore, it aims to expand our understanding of the conserved region of the VP1 protein.

Methods: Total samples (n=50) were subjected to antigen detection ELISA and RT-PCR for serotype determination. Confirmed serotype-O isolates (n=17) underwent sequencing for lineage comparison, mutation impact assessment on the VP1 protein GH loop, 3D structure prediction, and further comparative analysis.

Results: Isolates collected from 2017 to 2020 were identified as serotypes O/ME-SA/Pan Asia II ANT10 and O/ME-SA/Pak14. Notably, isolates collected from 2020 to 2022 belonged to a novel FMDV serotype O/ME-SA/Ind-2001e lineage. Phylogenetic analyses indicated that these strains were distinct from dominant contemporaneous strains which may challenge Pakistan's FMD control measures. These isolates exhibited variance in the VP1 epitope, specifically in amino acid residues 135-155, known to influence neutralizing antibody generation.

Conclusion: Observed mutations suggest potential challenges to current vaccination efficacy against FMD. This emphasizes enhanced FMD surveillance and demonstrates that tracking the emergence of the O/ME-SA/Ind-2001e lineage is important for determining FMD control strategies in Asia.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

巴基斯坦口蹄疫病毒血清型-O顶型 ME-SA/Ind-2001 的进化动态：2017-2022 年。

背景：目的：本研究对 Ind-2001 和泛亚 II 系进行了全面的进化分析，特别强调了它们在该地区的地理分布、系的分类和亚系的分布。此外，该研究还旨在扩大我们对 VP1 蛋白保守区的了解：方法：对总样本（n=50）进行抗原检测 ELISA 和 RT-PCR，以确定血清型。对确认的血清型-O分离株（n=17）进行测序，以进行品系比较、VP1蛋白GH环突变影响评估、三维结构预测和进一步比较分析：结果：2017年至2020年收集的分离株被鉴定为血清型O/ME-SA/泛亚II ANT10和O/ME-SA/Pak14。值得注意的是，2020年至2022年采集的分离株属于新型FMDV血清型O/ME-SA/Ind-2001e系。系统发育分析表明，这些毒株有别于同时代的优势毒株，可能对巴基斯坦的口蹄疫控制措施构成挑战。这些分离株在 VP1 表位上表现出差异，特别是在氨基酸残基 135-155 上，已知这将影响中和抗体的产生：结论：观察到的变异表明，目前的口蹄疫疫苗接种效果面临潜在挑战。结论：观察到的变异表明，目前的口蹄疫疫苗疗效可能面临挑战，这就需要加强口蹄疫监测，并表明跟踪 O/ME-SA/Ind-2001e 株系的出现对于确定亚洲的口蹄疫控制策略非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.