Omega-3 fatty acids supplementation prevents learning and memory impairment induced by chronic ethanol consumption in adolescent male rats through restoration of inflammatory and oxidative responses

IF 1.7 4区医学 Q3 DEVELOPMENTAL BIOLOGY International Journal of Developmental Neuroscience Pub Date : 2024-05-27 DOI:10.1002/jdn.10336

Murtaza Haidary, S. Mohammad Ahmadi-Soleimani, Mina Ghofraninezad, Hassan Azhdari-Zarmehri, Farimah Beheshti

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Abstract

Objective

Ethanol (Eth) intake is known to cause numerous detrimental effects on the structure and function of the brain, and it is commonly used as a psychostimulant drug by adolescents. Conversely, omega-3 (O₃) can reduce the risk of cognitive decline and promote the maintenance of neurophysiological functions. In this study, we investigated the protective effects of O₃ on behavioral alterations, oxidative stress, and interleukin-6 (IL-6) levels induced by chronic Eth intake during adolescence in rats.

Materials and methods

Adolescent male rats (21 days old) were divided as follows: (1) Vehicle, (2) Eth (Eth in drinking water [20%]), (3–5) Eth + O₃ (50/100/150 mg/kg), and (6) O₃ (150 mg/kg). After 5 weeks, Morris water maze (MWM) and passive avoidance (PA) tests were performed, and the hippocampal and cortical levels of oxidative stress markers and inflammatory indices were measured.

Results

Adolescent Eth intake impairs learning and memory function in MWM and PA tests (groups × day, p < 0.05 and p < 0.001, respectively). It was shown that Eth induced oxidative stress and neuroinflammation. O₃ improved learning and impairment induced by Eth by reducing the adverse effects of Eth on the oxidant/antioxidant balance in the hippocampi (for malondialdehyde [MDA]/thiol: p < 0.01, p < 0.001, respectively) and for superoxide dismutase (SOD)/catalase (CAT): p < 0.01 and p < 0.05, respectively). Furthermore, we found that O₃ prevented the Eth-induced increase of hippocampal IL-6 (p < 0.001).

Conclusion

O₃ supplementation acts as an effective approach to prevent learning and memory impairments induced by chronic Eth consumption during adolescence. In this respect, the antioxidant and anti-inflammatory properties of O₃ seem to be the main underlying mechanisms of neuroprotection.

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通过恢复炎症和氧化反应，补充奥米加-3 脂肪酸可预防青少年雄性大鼠因长期摄入乙醇而导致的学习和记忆损伤。

研究目的众所周知，摄入乙醇（Eth）会对大脑的结构和功能造成诸多不利影响，而且乙醇通常被青少年用作精神兴奋剂。相反，ω-3（O3）可以降低认知能力下降的风险，促进神经生理功能的维持。在这项研究中，我们调查了 O3 对大鼠青春期慢性乙醇摄入诱导的行为改变、氧化应激和白细胞介素-6（IL-6）水平的保护作用：将青春期雄性大鼠（21 天大）分为以下几组：(1) 车辆；(2) Eth（饮用水中的 Eth [20%]）；(3-5) Eth + O3（50/100/150 mg/kg）；(6) O3（150 mg/kg）。5周后，进行莫里斯水迷宫（MWM）和被动回避（PA）测试，并测量海马和大脑皮层的氧化应激标记物水平和炎症指数：结果：青少年摄入乙醇会损害MWM和PA测试中的学习和记忆功能（组别×日，p 3通过减少乙醇对海马氧化剂/抗氧化剂平衡的不利影响，改善乙醇引起的学习和记忆损害（丙二醛[MDA]/硫醇：p 3防止乙醇引起的海马IL-6的增加（p 结论：补充O3能有效改善海马氧化剂/抗氧化剂平衡，从而减少乙醇对海马氧化剂/抗氧化剂平衡的不利影响：补充 O3 是预防青春期长期摄入乙导致的学习和记忆障碍的有效方法。在这方面，O3 的抗氧化和抗炎特性似乎是神经保护的主要潜在机制。

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来源期刊

International Journal of Developmental Neuroscience 医学-发育生物学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： International Journal of Developmental Neuroscience publishes original research articles and critical review papers on all fundamental and clinical aspects of nervous system development, renewal and regeneration, as well as on the effects of genetic and environmental perturbations of brain development and homeostasis leading to neurodevelopmental disorders and neurological conditions. Studies describing the involvement of stem cells in nervous system maintenance and disease (including brain tumours), stem cell-based approaches for the investigation of neurodegenerative diseases, roles of neuroinflammation in development and disease, and neuroevolution are also encouraged. Investigations using molecular, cellular, physiological, genetic and epigenetic approaches in model systems ranging from simple invertebrates to human iPSC-based 2D and 3D models are encouraged, as are studies using experimental models that provide behavioural or evolutionary insights. The journal also publishes Special Issues dealing with topics at the cutting edge of research edited by Guest Editors appointed by the Editor in Chief. A major aim of the journal is to facilitate the transfer of fundamental studies of nervous system development, maintenance, and disease to clinical applications. The journal thus intends to disseminate valuable information for both biologists and physicians. International Journal of Developmental Neuroscience is owned and supported by The International Society for Developmental Neuroscience (ISDN), an organization of scientists interested in advancing developmental neuroscience research in the broadest sense.