Comprehensive analysis of changes in expression of lncRNA, microRNA and mRNA in liver tissues of chickens with high or low abdominal fat deposition.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE British Poultry Science Pub Date : 2024-06-01 Epub Date: 2024-05-29 DOI:10.1080/00071668.2024.2319779

S Yu, G Wang, X Shen, J Chen, J Liao, Y Yang, G Aikebai

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Abstract

1. The liver of chickens is a dominant lipid biosynthetic tissue and plays a vital role in fat deposition, particularly in the abdomen. To determine the molecular mechanisms involved in its lipid metabolism, the livers of chickens with high (H) or low (L) abdominal fat content were sampled and sequencing on long non-coding RNA (lncRNA), messenger RNA (mRNA) and small RNA (microRNA) was performed.2. In total, 351 expressed protein-coding genes for long non-coding RNA (DEL; 201 upregulated and 150 downregulated), 400 differentially expressed genes (DEG; 223 upregulated and 177 downregulated) and 10 differentially expressed miRNA (DEM; four upregulated and six downregulated) were identified between the two groups. Multiple potential signalling pathways related to lipogenesis and lipid metabolism were identified via pathway enrichment analysis. In addition, 173 lncRNA - miRNA - mRNA interaction regulatory networks were identified, including 30 lncRNA, 27 mRNA and seven miRNA.3. These networks may help regulate lipid metabolism and fat deposition. Five promising candidate genes and two lncRNA may play important roles in the regulation of adipogenesis and lipid metabolism in chickens.

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全面分析腹部脂肪沉积高或低的鸡肝脏组织中 lncRNA、microRNA 和 mRNA 的表达变化。

1.鸡的肝脏是主要的脂质生物合成组织，在脂肪沉积，尤其是腹部脂肪沉积中起着至关重要的作用。为了确定鸡肝脂质代谢的分子机制，对腹部脂肪含量高（H）或低（L）的鸡肝进行了采样，并对长非编码 RNA（lncRNA）、信使 RNA（mRNA）和小 RNA（microRNA）进行了测序。两组之间共鉴定出 351 个长非编码 RNA 蛋白编码基因（DEL：201 个上调，150 个下调）、400 个差异表达基因（DEG：223 个上调，177 个下调）和 10 个差异表达 miRNA（DEM：4 个上调，6 个下调）。通过通路富集分析，确定了与脂肪生成和脂质代谢相关的多个潜在信号通路。此外，还发现了 173 个 lncRNA - miRNA - mRNA 相互调控网络，包括 30 个 lncRNA、27 个 mRNA 和 7 个 miRNA。这些网络可能有助于调节脂质代谢和脂肪沉积。5个有希望的候选基因和2个lncRNA可能在鸡的脂肪生成和脂质代谢调控中发挥重要作用。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .